COMPARATIVE INVESTIGATON OF THE FATTY ACID COMPOSITION IN MILK THISTLE (SILYBUM) SEED OIL, SUNFLOWER (HELIANTHUS) SEED OIL, AND SEA BUCKTHORN (HIPPOPHAE) SEED OIL

Download full text PDF
DOI: https://doi.org/None
Issue: 
6
Year: 
2017

Professor V.A. Kurkin, PhD; D.V. Rosikhin; T.K. Ryazanova, PhD Samara State Medical University; 89, Chapaevskaya St., Samara 443099, Russian Federation

Introduction. The paper considers the identification of milk thistle (Silybum) seed oil, sunflower (Helianthus) seed oil, and sea buckthorn (Hippophae) seed oil in terms of their characteristic fatty acid profile. Objective: to investigate the fatty acid composition of milk thistle seed oil and sea buckthorn seed oil as compared to that of sunflower seed oil. Material and methods. The objects of the investigation were the fatty oil of Saint-Mary-thistle (Silybum marianum), the medicinal product «Sea Buckthorn Seed Oil», and the pharmaceutical substance «Sunflower Seed Oil». The fatty-acid composition of the oils was analyzed using gas chromatography-mass spectrometric detection according to the GOST 31665-2012 method. Results. 15, 14, and 13 fatty acids were identified in the oils of milk thistle, sunflower, and buckthorn seeds, respectively. There was a preponderance of unsaturated fatty acids in all examined samples and linoleic, oleic, palmitic, and palmitoleic acids in the sea buckthorn seed oil. Unsaturated fatty acids, such as linoleic and oleic ones, and saturated acids, such as stearic, palmitic, arachidic, and begenic ones, create the main profile of milk thistle seed oil on a chromatogram. The oils of Saint-Mary-thistle and sunflower seeds differ qualitatively in fatty acid composition and content. Conclusion. The qualitative composition and quantitative content of fatty acids were established in the test oils; their distinctive features were revealed. The findings can be used to confirm the identity of the oils.
Keywords: 
Saint-Mary-thistle
Silybum marianum (L.) Gaertn.
common sunflower
Helianthus annuus L.
sea buckthorn
Hippophae rhamnoides L.
fatty oil
fatty acids
composition
content
References: 
  1. Rosihin D.V., Kurkin V.A., Ryzhov V.M. Farmakognosticheskoe issledovanie po obosnovaniyu kompleksnogo ispol`zovaniya rastoropshi pyatnistoy. Aspirantskiy vestnik Povolzh`ya, 2015; 5–6 (2): 342–6.
  2. Samigullina L.I., Lazareva D.N. Novye perspektivy primeneniya preparatov rastoropshi pyatnistoy. E`ksperimental`naya i klinicheskaya farmakologiya, 2004; 67 (4): 77–80.
  3. Kurkin V.A. Rastoropsha pyatnistaya – istochnik lekarstvennyh sredstv (obzor). Himiko-farmacevticheskiy zhurnal, 2003; 37 (4): 27–41.
  4. Kurkin V.A. Farmakognoziya. Samara: Ofort, SamGMU. 2016; 1279.
  5. Deyneka V.I., Deyneka L.A., Sorokoludov V.N. Obrashhenno-fazovaya VE`ZhH v analize rastitel`nyh masel. Metod kontrolya podlinnosti i ustanovleniya fal`sifikacii oblepihovogo masla. Himiko-farmacevticheskiy zhurnal, 2009; 43 (1): 33–6.
  6. GOST 31665-2012. Masla rastitel`nye i zhiry zhivotnye. Poluchenie metilovyh e`firov zhirnyh kislot.
  7. Sokol`skaya T.A. Sozdanie lekarstvennyh sredstv iz plodov rastoropshi pyatnistoy (poluchenie, standartizaciya i kontrol` kachestva): dis. doktora farm. nauk. M., 2000; 79.
  8. EMA/HMPC/294188/2013 Committee on Herbal Medicinal Products (HMPC), Assessment report on Silybum marianum (L.) Gaertn., fructus, 2015.
  9. Ismaili S.A., Harhar H., Gharby S. et al. Chemical composition of two non-conventional oils in Morocco: Melia azadirachta and Silybum marianum (L.). Journal of Materials and Environmental Science, 2016; 7 (6): 2208–13.
  10. European Pharmacopoeia. 5-th ed. Rockville: United States Pharmacopoeial Convention. Inc., 2008; 2524.