TO PREPARE THE IMMOBILIZED CELL FORMULATIONS OF THE ANTICANCER DRUGS VINCRISTINE AND VINBLASTINE

Download full text PDF
DOI: https://doi.org/10.29296/25419218-2018-04-05
Issue: 
4
Year: 
2018

A.D. Khalakhakun, O.V. Trineeva, A.I. Slivkin, E.E. Chupandina Voronezh State University; 1, Universtitetskaya Sq., Voronezh 394006, Russian Federation

Introduction. Chemotherapy is still relevant in cancer practice. The potentials of old drugs are not completely exhausted. When developing the targeted transport of novel anticancer drugs and their further introduction into practice, it is particularly important to change their pharmacokinetics, which is the basis for the selection of dosage regimens. Objective: to investigate the possibilities of preparing the immobilized cell formulations of the anticancer drugs vincristine and vinblastine for their targeted transport in the body. Material and methods. The terpenoid indole alkaloids vincristine and vinblastine from different manufactures were studied. The modified hypoosmotic lysis method was used to prepare a packed red blood cell formulation of the drugs. The efficiency of drug encapsulation in red blood cells was established spectrophotometrically. Results. The findings suggest that there is a fairly close degree of including the studied substances in red blood cell carriers. The shelf-life of isolated red blood cells has an impact on the efficiency of including vincristine and vinblastine in red blood cells. Conclusion. The study has shown the possibility of preparing the immobilized cell formulations of the anticancer drugs vincristine and vinblastine for their targeted transport. But the existing technology for encapsulated drugs is accompanied by significant losses of a drug. Therefore, it is very important to develop a procedure to enhance the efficiency of encapsulation of vincristine and vinblastine.
Keywords: 
vincristine
vinblastine
efficiency of inclusion
red blood cell carriers
targeted transport of drug substances
References: 
  1. Zaborovskiy A.V., Gurevich K.G. Modelirovanie napravlennogo transporta lekarstvennyh veshhestv. Chast` I. Odnokratnoe vvedenie. Sibirskiy onkologicheskiy zhurnal, 2017; 1 (16): 59–65.
  2. Zaborovskiy A.V., Gurevich K.G. Modelirovanie napravlennogo transporta lekarstvennyh veshhestv. Chast` II. Mnogokratnoe vvedenie. Sibirskiy onkologicheskiy zhurnal, 2017; 16 (2): 36–41.
  3. Halahakun A.D. i dr. Ustanovlenie optimal`nyh usloviy dlya standartizacii vinkristina metodom spektrofotometrii. Materialy VI Mezhdunarodnoy nauchno-metodicheskoy konferencii «Puti i formy sovershenstvovaniya farmacevticheskogo obrazovaniya. Sozdanie novyh fiziologicheski aktivnyh veshhestv». Voronezh: VGU, 2016; 570–5.
  4. Ó’Fágáin C.,Cum-mins P.M., O’Connor B.F. Gel-Filtration Chromatography. «Protein Chromatography Methods and Protocols: Methods in Molecular Biology» (by ed. D. Walls, S.T. Loughran). Totowa, NJ: Humana Press, 2011; 25–33.
  5. Nikolayenko I.V et al. Preparation of highly purified human IgG, IgM , and IgA for immuniza-tion and immunoanalysis. Ukrainica Bioorganica Acta, 2005; 2: 3–11.
  6. Stratton F., Smith D.S., Rawlinson V.I. Value of gel filtration on Sephadex G-200 in the analysis of blood group antibodies. Journal of Clinical Pathology, 1968; 21 (6): 708–14.
  7. Ibragimov A.N., Bikmullin A.G., Sataeva D.A., Lopuhov L.V., Zaynullin L.I. Hromatograficheskie metody ochistki belkov. Kazan`: KFU, 2013;1–48.