DETECTION OF VERAPAMIL IN BIOLOGICAL FLUIDS

Download full text PDF
DOI: https://doi.org/None
Issue: 
2
Year: 
2015

L.L. Kvachakhia, PhD; Professor V.K. Shormanov, PhD Kursk State Medical University; 3, K. Marx St., Kursk 305041

A procedure was developed to quantify verapamil in blood and urine. Acetone was proposed as an isolating agent to be used for the extraction of verapamil from biological fluids. The authors showed that the test compound could be purified from the co-extractives of the biomaterial on a column packed with the sorbent Silasorb C-18 with a particle size of 30-μm. In doing this, the optimal eluate is a mixture of acetonitrile solvents and water (9:1). Thin layer chromatography, chromatography mass spectrometry, and UV-spectrophotometry were used to identify and quantify verapamil in the blood and urine extractions. The procedure can identify and determine 97.15 and 98.86% of verapamil in the blood and urine extractions, respectively. The minimally detectable amounts of verapamil in 100 g of these biofluids are 0.25 and 0.08 mg, respectively.
Keywords: 
verapamil
biological fluids
identification
quantification
References: 
  1. Kvachahiya L.L., Shormanov V.K. Identifikaciya nifedipina v biologicheskih zhidkostyah. Farmaciya, 2013; 62, (8): 16–19 [Quachakhia L.L., Shormanov V.K. Nifedipine identification in biological fluids. Farmatsiya. 2013; 62 (8): 16–19 (in Russian)].
  2. Mashkovskiy M.D. Lekarstvennye sredstva T.1. (Izd. 13). Har`kov: Torsing, 1998: 560 [Mashkovsky M.D. Medicines. Vol. 1. (13th edition). Kharkov: Torsing, 1998: 560 (in Russian)].
  3. Sedov A.T. Muzhanovskiy E`.B. Sudebno-himicheskoe dokazatel`stvo otravleniya verapamilom. Sudebno-medicinskaya e`kspertiza, 1980; 23, (2): 48–50 [Sedov A.I., Muzhanovsky E.B. Forensic-chemical evidence of verapamil poisoning. Sudebno-meditsinskaya expertiza. 1980; 23 (2): 48–50 (in Russian)].
  4. (+/-)-Verapamil hydrochloride. Chemical Properties,Usage,Production // Chemical Book. 2010. Mode of access: http://www.chemicalbook.com/ChemicalProductProperty_EN_CB7664827.htm.
  5. Satish Kumar K., Ravi Kumar K., Ravi Kumar D.V.V. et al. Validated new spectrophotometric methods for the estimation of verapamil in pharmaceutical dosage forms. J. Pharm. Educ. Res., 2010; 1, (2): 96–100.