EVALUATION OF THE EFFICACY OF A DRUG FROM URCHIN GONADS

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DOI: https://doi.org/None
Issue: 
2
Year: 
2015

I.E. Makarenko (1); N.M. Faustova (2), PhD; G.V. Vanatiev (1,2); I.N. Urakova (2), PhD; O.N. Pozharitskaya (2), PhD; M.N. Makarova (2), PhD; Professor V.G. Makarov (2), MD; A.N. Shikov (1,2), PhD 1 -I.I Mechnikov North-Western State Medical University; 41, Kirochnaya St., Saint Petersburg 191015 2 -Saint Petersburg Institute of Pharmacy; 27, Partizanskaya St., Office 424, Saint Petersburg 195248

The in vitro and in vivo biological effects of an antihyperglycemic drug derived from urchin (Strongylocentrotus droebachiensis) gonads. An investigation of dipeptidyl peptidase-4 (DPP-4) established the specific inhibitory activity of the test agent. IC50 was 20 μg/ml, which was similar to that of the synthetic DPP-4 inhibitors, such as the specific inhibitor S-EPC and the drug Sitagliptin (2.1 and 0.032 μg/ml, respectively); and greater than the activity of natural DPP-4 inhibitors (5% dry extracts from dandelion (Taraxacum officinale) and common nettle (Urtica dioica)). An investigation of male mice with experimental streptozotocin nicotinamide-induced diabetes mellitus showed that when used therapeutically (intragastrically for 14 days), the test drug produced a moderate glucose-lowering effect and an antioxidant one, which were similar to those of sitagliptin. The test urchin-derived drug was suggested to be not only a DPP-4 inhibitor, but also a GPR120 receptor agonist.
Keywords: 
dipeptidase-4
glucagone-like peptide-1
urchin
Strongylocentrotus droebachiensis
lipid extract
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