DRY BRIER (ROSA) PETAL EXTRACT: PREPARATION AND STANDARTIZATION

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DOI: https://doi.org/None
Issue: 
3
Year: 
2015

L.F. Zheznyakovskaya, PhD; A.A. Kubasova; Professor L.B. Okonenko, PhD Yaroslavl Mudryi Novgorod State University; 41, Bolshaya Sankt-Peterburgskaya St., Veliky Novgorod

A dry extract was prepared from brier (Rosa) petals. 1% hydrochloric acid and 20, 40, and 70% ethanol were used as extractants. The extracts were produced by remaceration and percolation at room temperature and 40°C; in the latter case, the yield of anthocyanins was maximal. Quantification of anthocyanins revealed that 70% ethanol was the optimal extractant to prepare a dry anthocyanin-containing extract from brier petals and percolation was the optimal method of extraction. The dry extract was standardized from the level of anthocyanins by spectrophotometry at a wavelength of 510 nm; that of heavy metals, and moisture. Sodium hydrochloride, 2% lead acetate, and hydrochloric acid were used to carry out identity tests. The dry anthocyanin-containing extract from rose cinnamomea (Rosa majalis) was proposed to prepare its formulations as an antioxidant, anti-inflammatory, and visual apparatus-protective agent in the treatment of diseases of various etiologies and as an adjunct in antitumor therapy.
Keywords: 
anthocyanins
dry extract
Rosa majalis L.
brier
rose cinnamomea
petals
References: 
  1. Gosudarstvennaya farmakopeya: SSSR XI izd. Vyp. 1, 2. M.: Medicina, 1987: 336 s; 1989: 400.
  2. Egorova A.V. Issledovaniya po standartizacii plodov rasteniy, soderzhashhih veshhestva antocianovoy prirody: Avtoref. dis. kand. farm. nauk. Samara, 2013: 24.
  3. Kubasova A.A., Zheznyakovskaya L.F. Podbor e`kstragenta dlya polucheniya suhogo e`kstrakta, soderzhashhego antociany, iz lepestkov shipovnika. Sbornik materialov IV Vserossiyskoy nauchnoy konferencii studentov i aspirantov s mezhdunarodnym uchastiem «Molodaya farmaciya – potencial budushhego», Sankt-Peterburg, 14–15 aprelya 2014 g. SPb.: SPHFA, 2014: 291–293.
  4. Pticyn A.V., Muhtarov E`.I., Kaplun A.P., Muhtarova S.E`. Sposob vydeleniya biologicheski aktivnyh antocianov. Pat. RF № 2302423, 2007zayav. 2005117999/04; zayavl. 06.10.2005; opubl. 2007.07.10
  5. Kowalczyk E., Krzesiński P., Kura M., Szmigiel B., Błaszczyk J. Anthocyanins in medicine. Pol. J. Pharmacol. 2003 Sep-Oct; 55(5): 699–702.
  6. Hou D.X., Ose T., Lin S., Harazoro K., Imamura I., Kubo M., Uto T., Terahara N., Yoshimoto M., Fujii M. Anthocyanidins induce apoptosis in human promyelocytic leukemia cells: structure-activity relationship and mechanisms involved. Int. J. Oncol. 2003; 23 (3): 705–712.