SPECIFIC CHARACTERISTICS OF ISOLATION OF SOME BACTERIAL SPECIES FROM MEDICATIONS

The paper shows that certain bacterial species may be isolated and differentiated during their co-cultivation in the testing for the index «Microbiological purity» in the presence and absence of a drug. The trend in E. coli, S. aureus, and P. aeruginosa growth on a nonselective liquid medium has been studied. The strains tested are noted to be competitive for the nutrient substrates of a storage medium, which is due to the specific features of the growth of lag-phase bacterial cultures. The issues of the possibilities and validity of reducing a sample for microbiological examinations are considered. A number of pharmaceutical substances are used as an example to show that test sample mass exerts no impact on the probability of identifying certain species of contaminant microorganisms. In particular, E.coli can be isolated and measured from both 10 and 1 g of a medication with the same level of accuracy, as suggested by the results of appropriate processing of the findings. There is evidence that the sample mass can be reduced for analysis and simultaneous isolation of certain microbial species regulated by State Pharmacopoeia 12th Edition.