SPECIFIC FEATURES OF THE DETERMINATION OF HYDROXYBENZOIC ACID IN BIOLOGICAL MATERIAL

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DOI: https://doi.org/None
Issue: 
2
Year: 
2016

V.K. Shormanov (1), PhD; V.V. Chupak (2), PhD; A.P. Astashkina (1), PhD; G.B. Golubitsky (3), PhD; N.A. Kibets (1); M.N. Pobedonostsva (1) 1 -Kursk State Medical University; 3, K. Marx St., Kursk 305041 2 -Oryol State University; 95, Komsomolskaya St., Oryol 302026 3 -OAO «Farmstandart-Leksredstva»; 1A/18, Second Agregatnaya St., Kursk 305022

An acetone-ethyl acetate (7:3) mixture was proposed as an insulator for the extraction of 2-hydroxybenzoic acid (2-HOBA) from biological objects. The optimal conditions for isolating 2-HOBA with this insulator from biological material were determined. The paper shows how the compound in question can be purified from the endogenous substances of the biomaterial through extraction in combination with liquid chromatography in a column packed with silica gel L 40/100 μm. Thin-layer chromatography, ultraviolet spectrophotometry, and high-performance liquid chromatography were used for the identification of 2-HOBA in the extracts from liver tissue and blood. The photometric isolation of 2-HOBA from organ tissues and blood was quantified using a reaction to obtain an acy-nitro derivative of the substances in question. The least detectable level of 2-HOBA in liver tissue, blood, and putrefactive liver tissue was 0.10, 0.08, and 0.12 mg per 100 g of the biomaterial, respectively.
Keywords: 
2-hydroxybenzoic acid
salicylic acid
isolation
identification
determination
biological material
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