THIN LAYER CHROMATOGRAPHY IN THE ANALYSIS OF AMARYLLIDACEAE ALKALOIDS

Download full text PDF
DOI: https://doi.org/None
Issue: 
7
Year: 
2016

D.O. Bokov; Professor I.A. Samylina, PhD I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

The paper considers the variants of thin layer chromatography (TLC) of Amaryllidaceae alkaloids that are a part of metabolome of 2 snowdrop species. It presents the component composition of mobile phases, the type of a sorbent as a stationary phase, and techniques for detecting the adsorption zones. Chloroform is the major component in the mobile phase used for TLC analysis of Amaryllidaceae alkaloids. TLC is shown to allow the identification of a mixture of Amaryllidaceae alkaloids, including galantamine and lycorine, and the use for standardization of raw homeopathic medicinal plant materials, homeopathic matrix tinctures of two snowdrop species.
Keywords: 
Amaryllidaceae alkaloids
galantamine
thin layer chromatography
References: 
  1. Bokov D.O., Samylina I.A. Himicheskiy sostav i primenenie v medicine podsnezhnika. Materialy III Nauch.-prakt. konf. «Sovremennye aspekty ispol`zovaniya rastitel`nogo syr`ya i syr`ya prirodnogo proishozhdeniya». Sechenovskiy vestnik, 2015;1 (19): 78–9.
  2. Proskurnina N.F., Yakovleva A.P. Ob alkaloidah Galanthus woronowii. Chast` II.O vydelenii novogo alkaloida. Zhurn. obshhey himii,1952; 22 (10): 1899.
  3. Bubeva–Ivanova L. Phytochemical in vestigation of Galanthus nivalis var. gracilis. Fram. pol., 1957; 7: 23-26.
  4. Paskov D.S. La Nivalina. Farmacologia e Applicazione. Clinica.. Bologna: Capelli.Editore., 1962; 84.
  5. Cherkasov O.A., Poshkurlat A.P., Stihin V.A. Soderzhanie galantamina v populyaciyah ungernii. Him.-farm. zhurn., 1984; 3: 327–9.
  6. Cherkasov O.A. Belocvetnik letniy – perspektivnyy galantaminosoderzhashhiy vid. Resursy dikorastushhih lekarstvennyh rasteniy SSSR. M.: VILAR. Minmedprom, 1975; 3: 238–41.
  7. Maysuradze N.I., Cherkasov O.A., Stihin V.A. List`ya narcissa kak syr`e dlya polucheniya galantamina. Him.-farm. zhurn., 1985; 19 (3): 190–2.
  8. Proskurnina N.F., Areshkina L.Ya. Ob alkaloidah Galanthus woronowii. Chast` I. Zhurn. obshhey himii, 1947;17 (6): 1266.
  9. Yakovleva A.P. Izuchenie alkaloidov podsnezhnika Voronova. Avtoref. diss. kand. him. nauk. M.,1964; 24.
  10. Briggs S.K., Highet P.F., Highet R.J., Wildman W.C. Alkaloids of the Amaryllidaceae. Part VII. Alkaloids containing the hemiacetol or lactone group. J.Amer.Cnem.Soc., 1956; 78 (12): 2899.
  11. Boit H.G. Über Amaryllidaceen-Alkaloide. Part III. Mitt. Über die Alkaloide der Zwiebeln von Galanthus nivalis. Chem.Ber.,1954; 87 (5): 724.
  12. Kalashnikov I.D. Issledovanie podsnezhnika belosnezhnogo, proizrastayushhego v zapadnyh oblastyah USSR, kak istochnika galantamina. Avtoref. diss. kand. farm. nauk. L`vov, 1970; 20.
  13. Sandberg F., Michel K.-H. Phytochemische Studienüber die Alkaloide von Pancratium maritimum. Lloydia, 1963; 26: 78–90.
  14. Asoeva E.Z., Vergeychik E.H. Razdelenie i izuchenie kolichestvennogo sostava alkaloidov podsnezhnika Krasnova. Nauchnye doklady vysshey shkoly. Biologicheskie nauki, 1967; 7: 98–101.
  15. Gorbunova G.M., Patudin A.B., Gorbunov V.D. Galantamin iz nekotoryh vidov semeystva Amaryllidaceae. Chast` II. Himiya prirodnyh soedineniy, 1978; 3 (14): 420–2.
  16. Leifertova I., Brazdova V. Alkaloids of Galanthus nivalis grownin Slovakian provinces. Ceskoslovenska Farmacie, 1967;16: 352–4.
  17. Stefanov Zh. Quantitative and qualitative study of the alkaloid composition of wild and introduced Leucojum aestivum L. populations. Part II. Method for the simultaneous quantitative determination of galanthamine and some of the major alkaloids in the above ground parts and bulbs of the plant. Farmatsiya (Sofia), 1977; 27: 4–10.
  18. Wurst F., Prey T., Puchinger L., Bancher E. Eine neue Methode zur quantitative Bestimmung von Galanthamin in Drogen extracten von Leucojum aestivum. Journal of Chromatography,1980; 188: 452–6.
  19. Tohtabaeva G.M. Hromatofotokolorimetricheskiy metod opredeleniya galantamina v liste narcissa sorta Fortune. Him.-farm. zhurn., 1987; 6: 703–5.
  20. Sadykov Yu.D., Hodzhimatov M. Soderzhanie alkaloidov v Ungernia victoris Vved. Tadzhikskoy SSR. Rastitel`nye recursy,1988; 24 (3): 410–4.
  21. Volodina A.D., Dobronravova E.K., Shakirov T.T. Spektrofotometricheskoe opredelenie likorina v rastitel`nom syr`e i preparate. Himiya prirodnyh soedineniy, 1972; 6 (8): 761–3.
  22. Li H.Y., Ma G.E., Xu Y., Hong S.H. Alkaloids of Lycoris guangxiensis. Planta Medica, 1987; 53: 259–61.
  23. Hong S.H., Li J.F., Xu R.X. Studies on alkaloids of Amaryllidaceae. Part VI. Identification of galanthamine and lycoramine by thin-layer chromatography. Chih Wu Hsueh Pao, 1981; 23: 334–7.
  24. Dobronravova E.K., Sattarova A.H., Shakirov T.T. Hromatograficheskoe povedenie galoidnyh soley alkaloidov na okisi alyuminiya. Himiya prirodnyh soedineniy, 1982; 1: 127–9.
  25. Volodina A.D., Dobronravova E.K. Polyarograficheskoe opre delenie galantamina Ungernia victoris. Himiya prirodnyh soedi-
  26. neniy, 1970; 4: 450–3.