Dry leaf extract of Lophantus anisatum Benth: obtaining and analysis of phenolic compounds

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DOI: https://doi.org/10.29296/25419218-2019-02-05
Issue: 
2
Year: 
2019

E.A. Iurtaeva(2), I.P. Remezova(1), A.G. Tyrkov(3), S.A. Luzhnova(2), O.I. Popova(1) 1-Рyatigorsk Medical Pharmaceutical Institute of Volgograd Medical State University of the Ministry of Health Care of Russia; 11, Kaninina Pr., Рyatigorsk 357532, Russian Federation, 2-Leprosy Research Institute; 3, N. Ostrovskogo Ln., Astrakhan 414057, Russian Federation, 3-Astrakhan state University; 1, Shaumiana Sq., Astrakhan 414000, Russian Federation

Introduction. Lophantus anisatum Benth. is a plant introduced by the Astrakhan region. The plant contains essential oil, flavonoids, tannins, triterpene acids, organic acids, ascorbic acid and is a valuable raw material for effective medicines. Objective: development of a method for producing dry leaf extract of Lophantus anisatum using supercritical fluid extraction and studying the chemical composition of its phenolic compounds. Materials and methods. The leaves of Lophantus anisatum are provided by the experimental farm of the State Russian Research Institute of Irrigated Melon-Growing of the Russian Academy of Agricultural Sciences. Leaf extract was obtained by supercritical fluid extraction. The composition of the phenolic compounds of the extract was studied by high performance liquid chromatography and mass spectroscopy (HPLC-MS). Results. Gallic acid, coumarin, umbelliferone, luteolin, quercetin, taxifolin, rutin are identified in the dry extract of Lophantus anisatum leaves. The main ones are luteolin (47.80%), rutin (2.57%), quercetin (3.61%). The content of the total flavonoids in terms of luteolin in the extract leaves 58.45-60.90%. The extract contains 0,85–0,87% tannins. Conclusion. An optimal method has been developed for the preparation of Lophantus anisatum leaf extract using supercritical fluid extraction and the composition of phenolic compounds in the resulting extract has been studied.
Keywords: 
Lophantus anisatum Benth.
supercritical fluid extraction
phenolic compounds
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