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Effect of dimethyl sulfoxide on the magnitude of the anti-inflammatory and analgesic activities of hymantane gels

DOI: https://doi.org/10.29296/25419218-2019-06-07
Issue: 
6
Year: 
2019

A.I. Matyushkin(1), E.A. Ivanova(1), T.A. Voronina(1), E.V. Blynskaya(1), K.V. Alekseev(1), A.I. Marakhova(2) 1-V.V. Zakusov Research Institute of Pharmacology, 8, Baltiyskaya St., Moscow 125315, Russian Federation; 2-Peoples’ Friendship University of Russia; 6, Miklukho-Maklai St., Moscow 117198, Russian Federation

Introduction. Dimethyl sulfoxide (DMSO) is widely used to improve the penetration of topical drugs into tissues, allowing their therapeutic dose to be reduced. This property of DMSO has become one of the reasons for incorporating it as an absorption activator into the composition of hymantane gels for topical application. Objective: to evaluate the analgesic and anti-inflammatory activities of hymantane gels with compositions characterized by the presence of the absorption activator DMSO. Material and methods. The investigation objects were 5% hymantane gels and placebos of various compositions. The analgesic activity of the gels was evaluated in a formalin test on male rats. Results. 5% hymantane gels were ascertained to have a comparable analgesic effect against pain caused by an inflammatory process. Hymantane gel containing DMSO as an absorption activator reduced the intensity of acute-phase formalin pain characterized by the effect of algogen on primary afferents of pain and showed anti-inflammatory activity comparable to that of 1% sodium diclofenac gel. Conclusion. Addition of DMSO as an absorption activator to 5% hymantane gel enhances the analgesic and anti-inflammatory effects of the active ingredient.

Keywords: 
gels for topical application
absorption activator
formalin pain
formalin-induced edema

References: 
  1. Patent of Enhancing tissue penetration of physiologically active agents with DMSO. US 3551554 A.
  2. Semkina O.A., Dzhavahjan M.A., Levchuk T.A.i dr. Vspomogatel'nye veschestva, ispol'zuemye v tehnologii mjagkih lekarstvennyh form (mazej, gelej, linimentov, kremov). Himiko-farmatsevticheskij zhurnal, 2005; 39 (9): 45–8. [Semkina O.A., Dzhavakhyan M.A., Levchuk T.A. et al. Auxiliary substances used in the technology of soft dosage forms (ointments, gels, linimentov, creams). Khimiko-farmatsevticheskiy zhurnal, 2005; 39 (9): 45–8 (in Russian)].
  3. Spravochnik Vidal' 2015. Lekarstvennye preparaty v Rossii. Vidal 2015. [Directory Vidal 2015. Medications in Russia. Moscow, Vidal 2015 (in Russian)].
  4. Ivanova E.A., Kapitsa I.G., Nepoklonov A.V. i dr. Protivovospalitel'naja aktivnost' gimantana na modeljah perifericheskogo vospalenija i nejrovospalenija, indutsirovannogo lipopolisaharidom. Himiko-farmatsevticheskij zhurnal, 2013; 10: 2–15. [IvanovaE.A., KapitsaI.G., NepoklonovA.V. et al. Anti-inflammatory activity of himantane on models of peripheral inflammation and neuroinflammation induced by lipopolysaccharide. Khimiko-farmatsevticheskiy zhurnal, 2013; 10: 2–15 (inRussian)].
  5. Ivanova E.A., Kapitsa I.G., Valdman E.A., Voronina T.A. Comparison of the analgesic activity of antiparkinsonian aminoadamantane derivatives amantadine and hemantane. Advances in Parkinson’s Disease, 2016; 3: 50–60.
  6. Il-Ok Lee, You-Seong Jeong. Effects of different concentrations of formalin on paw edema and pain behaviors in rats. J. Korean. Med. Sci., 2002; 17: 81–5.
  7. Voronina T.A., Guzevatyh L.S. Metodicheskie rekomendatsii po izucheniju anal'geticheskoj aktivnosti lekarstvennyh sredstv. Rukovodstvo po provedeniju doklinicheskih issledovanij lekarstvennyh sredstv. Chast' pervaja. M.: GrifiK, 2012; 197–218. [Voronina T.A., Guzevatikh L.S. Guidelines for the study of the analgesic activity of drugs. Guidelines for conducting preclinical studies of drugs. Part 1. Moscow: GrifiK, 2012; 197–218 (in Russian)].
  8. Brown J.H., Kissel J.W., Lish P.M. Studies on the acute inflammatory response. I. Involvement of the central nervous system in certain models of inflammation. J. Pharmacol. Exp. Ther., 1968; 160: 231–42;
  9. LeeI. O., Crosby G. Halothane effect on formalin-induced paw edema and flinching in rats. J. Korean. Med. Sci. 1999; 14: 34–8