Use of fluorescence staining technique for identification of microorganisms in pharmaceutical analysis

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DOI: https://doi.org/10.29296/25419218-2019-08-01
Issue: 
8
Year: 
2019

O.V. Gunar, N.G. Sakhno, M.V. Roshchina Research Center for Examination of Medical Products, Ministry of Health of the Russian Federation; 8, Petrovsky Boulevard, Build. 2, Moscow 127051, Russian Federation

Standard techniques for the detection and quantification of bacteria and fungi from drugs are not adequate for rapid (less than 24 hours) assessment of injured or uncultivated microorganisms and for their isolation. The paper considers alternative methods for the direct determination of microbial cells in a sample, by using fluorescence staining at the key stage, for example, up-to-date technologies, such as microscopy and flow and solid-phase cytometry, which have not currently found a wide utility in the practice of pharmaceutical analysis due to methodological problems. It describes different fluorochromes widely used for scientific and practical purposes, the specific features and limitations of their application and gives a review of physiological and taxonomic fluorescent dyes. Emphasis is placed on the importance and prospects of fluorescence staining of samples under study in combination with current developing technologies; it is concluded that the application of different fluorophores requires careful selection of conditions and development and validation of procedures for analysis in practice.
Keywords: 
pharmaceutical analysis
identification of microorganisms
fluorescence staining
fluorochrome
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