Features of determination of cefpirome in biological material

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DOI: https://doi.org/10.29296/25419218-2021-01-08
Issue: 
1
Year: 
2021

A.A. Bez’yazychnaya, V.K. Shormanov, L.E. Siplivaya, M.V. Rymarova, A.V. Kukureka Kursk State Medical University, 3, Karl Marx St., Kursk 305004, Russian Federation

Introduction. Cefpirome is white to slightly yellow hygroscopic, crystalline powder, freely soluble in water. It belongs to the group of third-generation cephalosporin antibiotics and has a broad spectrum of action. Objective: to develop and validate procedures for determining cefpirome in biological material. Material and methods. Cefpirome was isolated with solvents of different chemical nature of the mixture models with liver tissue. The duration of isolation, its multiplicity, and the mass ratio of the isolating agent to the biological object were examined for its impact on the percentage of extraction of the test compound with an optimal isolating agent. Low-pressure macrocolumn reversed-phase liquid chromatography was used as a purification procedure. Identification and quantification were done by reverse-phase HPLC. The determination methods were validated according to well-known parameters. Results. An acetone-water (5:5) mixture was the most suitable isolating agent for extracting cefpirome from biological material. The necessary frequency of isolation of the analyte of ths mixture was 2; the duration of each contact of the isolating mixture with the biological object was 30 min; the isolating mixture/biomatrix ratio was 2:1. A procedure was developed to determine cefpirome in cadaveric organ tissues by reversed-phase HPLC, which involves preliminary isolation of the analyte with an acetone-water (5:5) mixture and its purification in a 150×10-mm macrocolumn containing Silasorb C8 sorbent with a particle size of 15 µm. The developed procedure was validated according to the criteria of specificity, linearity, accuracy, and precision. Conclusion. The results of the investigations make it possible to present the nature of isolation of cefpirome from biological material. The developed procedure provides extraction, purification, identification, and quantitative determination of cefpirome in biological material.
Keywords: 
cefpirome
isolation and purification
identification and quantification
low-pressure macrocolumn chromatography
HPLC
validation
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