Evaluation of the quality and biological activity of BioDihydroquercetin

DOI: https://doi.org/10.29296/25419218-2022-08-01
Issue: 
8
Year: 
2022

1-LLC“Sibpribor” the Russian Federation, St. Trilissera, 87, Irkutsk, 664047, Russian Federation;
2-Buryat scientific center, Siberian branch of the Russian Academy of Sciences, street Casanovas, 8, Ulan-Ude, 670047,
Russian Federation;
3-Krasnoyarsk state medical University named after Professor V.F. Voyno-Yasenetsky, Partizan Zheleznyak str., 1, Krasnoyarsk, 660022 Russian Federation

Introduction. BioDihydroquercetin is a natural dietary supplement made from larch wood, produced by “SibPribor” LLC using a proprietary technology under the trade mark “Baikal Legend”. BioDihydroquercetin powder is obtained with a low density and high rates of biological activity and bioavailability, accessibility. Sublingual administration ensures rapid and complete penetration of the biologically active substance into the blood. Objectives: a comprehensive study of the composition and the biological activity of BioDihydroquercetin in connection with the peculiarities of its production technology. Material and methods. The content of dihydroquercetin (%) was determined according to GOST 33504-2015 and pharmacopoeial monograph of the company "Taxifolia", the qualitative composition was studied using high-performance liquid chromatography. The antiradical activity of the powder to organic and inorganic radicals of natural and artificial origin was studied by spectrometry and coulometry to determine the biological activity of BioDihydroquercetin. Antioxidant potential was assessed using spectrophotometry and fluorimetry with the determination of the total antioxidant potential and the ability to absorb oxygen radicals. The iron-reducing and iron-chelating potential of BioDihydroquercetin was determined by spectrophotometry. The study of bioavailability was carried out using GIT model. In addition, the protection of the organic substrate from oxidative stress caused by lipid peroxides, as well as the anti-inflammatory and membrane-stabilizing potential, were studied by spectrophotometry. Results and discussion. The content of dihydroquercetin in the samples was 99.2–104%. The content of dihydroquercetin according to the HPLC method is 96.84±2.03. The product of high purity, 100% consists of an active substance, an accompanying satellite of dihydroquercetin is a natural accompanying satellite of dihydroquercetin – dihydrokaempferol, the content of which does not exceed 1%. Biodihydroquercetin was shown to have antioxidant, antiradical and iron-reducing activity. The conducted studies have shown that Biodihydroquercetin is effective for protecting the biological substrate. In addition, BioDihydroquercetin has shown to be a good inactivator of nitric oxide molecules and has a pronounced inhibitory effect on the albumin coagulation process, which indicates its high anti-inflammatory activity. BioDihydroquercetin also has a pronounced membrane stabilizing potential. The special form of the powder, produced according to the author's technology, ensures high bioavailability. Conclusion. BioDihydroquercetin is a highly active natural product, characterized as an effective antioxidant, created using an author's technology. Its high biological activity and purity indicate its effectiveness and safety, which allows it to be recommended to a wide range of the population as an antioxidant, anti-inflammatory, membrane-stabilizing and membrane-protective agent of a high degree of protection. Biodihydroquercetin can be recommended to healthy people, living in big cities as a means of preventing “diseases of civilization”, also to all people for active longevity. Besides, Biodihydroquercetin can be used as an adjuvant in complex therapy of cardio-vascular, oncological, neurological diseases as well as diabetes mellitus type 2.
Keywords: 
BioDihydroquercetin
dihydroquercetin
author's production technology
biologically active additive
natural antioxidant
anti-inflammatory effect
membrane protector
membrane stabilizer.
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