Microscopic signs of the raw material of shrubby cinquefoil

DOI: https://doi.org/10.29296/25419218-2023-05-02
Issue: 
5
Year: 
2023

L.G. Dvornikova, O.N. Mazko, S.G. Kurbatova, A.S. Boyko
Altai State Medical University, Lenin Ave, 40, Barnaul, 656038, Russian Federation

Introduction. Shrubby cinquefoil (Pentaphylloides fruticosa (L) O. Schwarz) has a large raw material base in Altai and is actively used for the production of one-component tea drinks and herbal collections, which are the object of tourist trade in the Altai Territory and the Altai Republic. Currently, the State Pharmacopoeia of the Russian Federation does not have the pharmacopoeial article for this type of plant raw material, and, therefore, there are no standardized quality indicators. Objective: determination of anatomo-diagnostic features of shoots of shrubby cinquefoil for the development of a pharmacopoeial article on this type of plant raw material. Material and methods. Five series of dried shredded plant raw material of shrubby cinquefoil produced by different enterprises of Altai Krai served as objects of the study. Microscopic studies were carried out using Micros Austria MC300 microscope and Microscope Digital Eyepiece MDC-560 digital camera. Results. The epidermal cells of the leaflet are polygonal, with straight lateral walls, a distinct thickening and rare anomocyte-type stomata. The leaflet surface, leaf plate margin and veins are covered with simple unicellular hairs; bicellular and cephalic hairs occur. When the leaf is bleached with 5% sodium hydroxide solution, the contents of the cells turn brownish-red. Petal epidermis has polygonal cells with thick walls; gynoecium densely hairy, simple hairs long. The fruit is densely covered with simple unicellular hairs; the parenchyma of the fruit contains inclusions of calcium oxalate in the form of drusen. Epidermal cells of shoots consist of thin-walled rectangular cells. Conclusion. In the course of the conducted work anatomo-diagnostic features of dried plant raw material of shrubby cinquefoil were established: epidermis cells of the leaflet polygonal with clear-shaped thickening, anamocytic type of the stomatal complex, numerous simple hairs consisting of a short basal cell and a long terminal cell, cephalic hairs, cells with brown content.
Keywords: 
Kuril bush tea
shrubby cinquefoil
microscopic examination
anatomo-diagnostic features
standardization.
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