Determination of flavonoids in the herb of purple passionflower (Passiflora incarnata)

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DOI: https://doi.org/10.29296/25419218-2019-01-03
Issue: 
1
Year: 
2019

M.D. Guseinov(1), A.A. Svistunov(2), V.V. Tarasov(2), N.V. Bobkova(2), D.O. Bokov(2) 1-Dagestan State Medical University; 1, Lenin Sq., Makhachkala 367000, Republic of Dagestan, Russian Federation; 2-I.M. Sechenov First Moscow State Medical University (Sechenov University); 8, Trubetskaya St., Build. 2, Moscow 119048, Russian Federation

Introduction. Purple passionflower (Passiflora incarnata) is introduced in the Russian Federation and grows along the Black Sea coast of the Caucasus. Purple passionflower herb is a constituent of sedative drugs, and it is also used in homeopathy. The plant has a complex composition of biologically active substances. Flavonoids are one of the groups that determine its pharmacological activity. Objective: to investigate the composition and content of flavonoids in purple passionflower herb. Material and methods. The investigation object is dried purple passionflower herb. Tests used an Agilent 1100 liquid chromatograph with a diode-array detector. Results. HPLC-UV analysis established that the purple passionflower contained 20 flavonoid compounds. There were 8 flavonoids: isovitexin, vitexin, rutin, luteolin, hyperoside, kaempferol, kaemferitrin, orientin, and isoorientin. The content of vitexin was 0.202±0.001%; the amount of flavonoids calculated with reference to vitexin was 0.891±0.015%. Conclusion. The content and composition of flavonoids in purple passionflower were established by a HPLC-UV method. The findings can be used to improve regulatory documentation for purple passionflower herb.
Keywords: 
purple passionflower (Passiflora incarnata L.)
standardization
flavonoids
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