The Journal is included in Russian and International Library and Abstract Databases
Russain Science Index (Russia)
DOI Registration Agency (USA)
Scientific Indexing Services (USA)
CAS Source index (USA)
Web of Science Group (USA)



E.A. Kupriyanova(1), V.A. Kurkin(1), V.M. Ryzhov(1), A.V. Lyamin(1), O.V. Kondratenko(1), A.V. Pomogaibin(2), V.B. Braslavsky(1) 1-Samara State Medical University, 89, Chapaevskaya St., Samara 443099, Russian Federation;2-Acad. S.P. Korolev Samara National Research University, 34, Moskovskoe Shosse, Samara 443086, Russian Federation

Introduction. Searches for drugs are currently underway to treat cystic fibrosis, a disease that is caused by a gene mutation and is characterized by impaired secretion by exocrine glands in vital organs. Patients infected with Burkholderia strains that are resistant to many currently available antibacterial drugs present the greatest problem for the therapy of cystic fibrosis. The promising source of biologically active compounds is black poplar (Populus nigra L.), the buds of which have a pronounced antibacterial effect.Objective: to comparatively investigate the antibacterial activity of black poplar bud and sprout tinctures in ethanol at various concentrations against the strains of cystic fibrosis. Material and methods. The objects of the investigation were tinctures (1:5) of black poplar buds and sprouts in 40, 70, 80, and 96% ethanol. The minimal inhibitory concentration (MIC) was estimated using two-fold serial dilutions in a broth medium. Clinical strains, such as Burkholderia cenocepacia ST, Burkholderia multivorans, Pseudomonas aeruginosa, from patients with cystic fibrosis served as test cultures for determining the antimicrobial activity of the tinctures. The comparison objects were ethanol at various concentrations and working standard sample solution of pinostrobin isolated from black poplar buds.Results. Poplar sprout tinctures were found to have a wide spectrum of bacteriostatic activity against all the strains tested. The samples studied had marked bactericidal and bacteriostatic activities. MIC against Burkholderia multivorans was found in the poplar bud tincture in 80% ethanol. All the tinctures tested showed a low antimicrobial activity against Pseudomonas aeruginosa.Conclusion. The results of the investigation can serve as a rationale for designing new drugs based on black poplar buds and sprouts as an agent for the treatment of cystic fibrosis.

cystic fibrosis
black poplar
Populus nigra L.
poplar buds
poplar sprouts
antibacterial activity

  1. Kondratenko O.V. Features of biological properties and factors of pathogenicity of non-fermenting gram-negative bacte-ria isolated from patients. Aspirantskii vestnik Povolzh’ia. Samara, 2012;1-2: 251–3 (in Russian).
  2. Nikitina N.V., Kuleshova S.A. A study of the pharmacolog-ical action of an ointment with an extract of black poplar buds. Fundamentalnye issledovaniya. Penza, 2011; 11–3: 554–8 (in Russian).
  3. Lyamin A.V., Botkin E.A., Baisheva G.M., Veretelnik E.N., Yakunina S.V. Characteristics of the antimicrobial effect of con-densate of tarry substances obtained from wax ear candles. Meditsinskiy almanakh, 2014; 2 (32): 78–81 (in Russian).
  4. Kurkin V.A., Braslavsky V.B., Ryzhov V.М. Species of the family Salicaceae – a promising source of antimicrobial and anti-inflammatory drugs. Sechenovskii vestnik, 2016; S1: 60–1 (in Russian).
  5. The definition of the sensitivity of microorganisms to anti-bacterial drugs. Guidelines. MUK 4.2.1890-04. Klinicheskaya mik-robiologiya i antimikrobnaya khimioterapiya, 2004; 6 (4): 306–59 (in Russian).