EFFECTS OF A ZINC-CONTAINING BIOCOMPOSITE ON THE PARIETAL MICROFLORA OF THE COLON

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DOI: https://doi.org/10.29296/25419218-2019-04-08
Issue: 
4
Year: 
2019

A.D. Kim(2), S.A. Lepekhova(1), E.V. Koval(2, 4), Ya.A. Kostyro(3), N.N. Trofimova(3), E.Yu. Chashkova(2), K.A. Apartsin1, 2, Yu.I. Pivovarov(2) 1-Irkutsk Research Center, Siberian Branch, Russian Academy of Sciences, 134, Lermontov St., Irkutsk 664033, Russian Federation; 2-Irkutsk Research Center of Surgery and Traumatology, 1, Bortsov Revolutsii St., Irkutsk 664003, Russian Federation; 3-A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences, 1. Favorsky St., Irkutsk 664033, Russian Federation; 4-Irkutsk State Medical Academy of Postgraduate Education, 100, Yubileinyi Microdistrict, Irkutsk 664079, Russian Federation

Introduction. The development of intestinal dysbiosis is one of the components in the pathogenesis of inflammatory bowel diseases. A zinc-containing biocomposite (L15) has been designed, which is a hydrophobic rectal ointment. Its composition contains a zinc-containing metal complex dihydroquercetin derivative that has antimicrobial and prebiotic properties. Objective: to assess the qualitative and quantitative changes in the composition of the parietal microflora of the animal colon after rectal administration of the zinc-containing biocomposite in the presence of induced ulcerative colitis. Material and methods. The study was conducted on 54 male Wistar rats divided into 2 groups. Ulcerative colitis was induced by the original procedure. The animals underwent general clinical observation and determination of the quantitative and qualitative composition of the parietal microflora of the distal colon. Results. The rectal administration of a zinc-containing biocomposite to the animals with simulated ulcerative colitis after 10 days led to maintenance of a high level of the representatives of obligate bacteria, such as Bifidobacterium spp. and Lactobacillus spp., which was 47.6% each in progressive disorders with the development of dysbiosis in the parietal microflora of the colon in the control group. Conclusion. In all experimental periods, the changes in the qualitative and quantitative composition of the parietal microflora of the colon in the experimental animals treated with the test zinc-containing biocomposite for a simulated ulcerative lesion of the colon mucosa were positive compensatory as a significant increase in the concentration of Bifidobacterium spp. at 10 days of the experiment and proceeded without the opportunistic microflora.
Keywords: 
ulcerative colitis
microflora
zinc-containing biocomposite
rectal ointment
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