Study of microdiagnostic signs of sea buckthorn leaves


N.A. Kovaleva, O.V. Trineeva, A.A. Gudkova, A.I. Kolotneva, D.K. Nosova
Voronezh State University, Universitetskaya pl., 1, Voronezh, 394018, Russian Federation

Introduction. The development of new herbal medicinal products requires a constant expansion of the raw material base with both new non-pharmacopoeial medicinal plants and nontraditional morphological groups of raw materials of already highly researched species. The introduction of such herbal substances into medical practice requires their standardization so that it can be possible to obtain and sell herbal medicinal products based on that substances. Thus, this necessitates the development of Product specification files. Objective: to study the microdiagnostic traits of sea buckthorn leaves to complete the section “Microscopy” in the draft of pharmacopoeial monograph for these types of herbal substances. Material and methods. The object of the study is the dried leaves of sea buckthorn (Hippophaë rhamnoides L.) harvested on the territory of the Voronezh region (Ostrogozhsky district) during the period of technical maturity of the fruits. Harvesting and drying of leaves was carried out in accordance with the general pharmacopoeial monograph “Leaves”. Sample preparation of raw materials was carried out using various techniques. Microscopy was carried out according to the general pharmacopoeial monograph “Techniques of microscopic and microchemical research of herbal substances and herbal medicinal products” of State Pharmacopeia (XIV edition) of the Russian Federation by light microscopy on a Biomed 6 microscope (Russia) using a Livenhuk C310 NG video camera (PRC) and Top View software (×86). Results. The main microdiagnostic traits of whole leaves of sea buckthorn have been revealed (reticulated venation; 3 types of trichomas: scutellate, stellate and scutellate-stellate; anomocytic type of stomatal complex (on the underside of the leaf); polygonal cells of the epidermis on the upside and cells of the epidermis with strongly branched walls on the underside of the leaf). Biometric characteristics of the main microdiagnostic traits are also determined. Conclusion. Optimal methods of microscopic examination of sea buckthorn leaves have been selected. Anatomical and diagnostic traits of sea buckthorn whole dried leaves also were established.

sea buckthorn,
Hippophaë rhamnoides L.
microscopic analysis
stomatal index
stellate and scutellate-stellate trichomas

  1. Mikheev A.M., Demenko V.I. Sea buckthorn. M.: Rosagropromizdat, 1990; 48. ISBN 5-260-02610-1 (in Russian).
  2. Vidal Handbook «Medicines in Russia» [Electronic edition]. Access mode: (in Russian).
  3. Pavlova A.B., Chirkina T.F. The method of using woody sea buckthorn greens in the food industry. Modern high-tech technologies. 2005; 5: 57–8 (in Russian).
  4. Imasheva N.M., Elina V.V., Sadomtseva O.S. et al. Preparation of dietary supplements based on vegetable raw materials. International Academic Bulletin. 2014; 3 (3): 52–5 (in Russian).
  5. Abdykalikova K.A., Nechiporenko L.P. Phytochemical composition of the aboveground part of sea buckthorn. Bulletin of the KSPI. 2008; 4: 104–7 (in Russian).
  6. Aituarova A.Sh., Zhusupova G.E. Isolation of biologically active substances from the aboveground part of the plant species Hippophae rhamnoides L. and the possibilities of their use in medicine. Bulletin of the Kazakh National Medical University. 2016; 3: 195–7 (in Russian)
  7. Melnikov O.M., Vereshchagin A.L., Koshelev Yu.A. Investigation of biologically active compounds of buds and leaves of male sea buckthorn plants. Chemistry of plant raw materials. 2010; 2: 113–6 (in Russian).
  8. Pop, Raluca Maria et al. Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties. Food chemistry. vol. 2014; 147: 1–9. DOI:10.1016/j.foodchem.2013.09.083.
  9. Pavlova A.B., Chirkina T. F., Zolotareva A.M. Biologically active food additive based on woody sea buckthorn greens. Chemistry of plant raw materials. 2001; 4: 73–6 (in Russian).
  10. Ibrahimov Z.R., Gaitova T.R. Sea buckthorn leaves as a source of BAS. Actual problems of chemistry, biology and biotechnology: materials of the X All-Russian Scientific Conference: North Ossetian State University named after K.L. Khetagurov. 2016; 323–5 (in Russian)
  11. Chirkina T.F., Zolotareva A.M., Plastinina Z.A. Promising plant sources of biologically active substances in the Baikal region. Equipment and technology of food production. 2009; 1 (12): 71–4 (in Russian).
  12. Chernyak D.M., Titova M.S. The content of carotene and vitamins E and C in Far Eastern plants. TMJ. 2015; 2 (60): 92–3 (in Russian)
  13. Nasudari A.A., Bagirov I.M. Study of sea buckthorn growing and cultivated in Azerbaijan for the production of medicines. Abstracts of the report of the scientific conference dedicated to the 100th anniversary of the birth of Academician M. Topchibashev. Baku, 1995; 416 (in Russian)
  14. Trineeva, O.V., Slivkin A.I. Samylina I.A. Issledovaniya po razrabotke proektov farmakopeinykh statei na plody i maslo oblepikhi krushinovidnoi. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2016; 3: 126–33 (in Russian)
  15. State Pharmacopoeia of the Russian Federation XIV ed. [Electronic resourse]. Access mode: (in Russian)
  16. Attrey, Dharam Paul et al. Pharmacognostical Characterization & Preliminary Phytochemical Investigation of Seabuckthorn (Hippophae rhamnoides L.) Leaves. Indo Global J. of Pharmaceutical Sciences. 2012; 2 (2): 108–13
  17. Samylina I. A., Anosova O.G. Farmakognoziya. Atlas: uchebnoe posobie: v 3-kh tomakh. M: GEOTAR-Media, 2010; 1: 188. ISBN 978-5-9704-1576-4 (in Russian)
  18. Potanina O.G., Samylina I.A. Farmakopeinye trebovaniya k mikroskopicheskomu analizu lekarstvennogo rastitel'nogo syr'ya. Farmatsiya. 2015; 4: 47 (in Russian)