DETERMINATION OF FLAVONOIDS IN YELLOW SWEET CLOVER (MELILOTUS OFFICINALIS) HERB

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DOI: https://doi.org/None
Issue: 
1
Year: 
2017

T.V. Shchemelinina ; Professor A.A.Sorokina, PhD I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

Introduction. Yellow sweet clover (Melilotus officinalis) is distributed throughout the European part of Russia. This herb is part of Nervine Tea Three and it is also used in homeopathy. The plant has a complex composition of biologically active substances, which accounts for its pharmacological activity. Objective. To investigate the composition and content of flavonoids in yellow sweet clover herb. Material and methods. The investigation was concerned with the dried yellow sweet clover herb harvested in the Moscow Region in the summer of 2014. This used Agilent 1100 HPLC with a spectrophotometric diode-array detector. Results. The presence of flavonoids in the yellow sweet clover herb was confirmed by qualitative reactions using thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). The raw material contains 1.17% of the amount of flavonoids represented by 11 compounds, mainly as glycosides, such as kaempferol and quercetin. Conclusion. The content and composition of flavonoids were established in the yellow sweet clover herb grown in the Moscow Region.
Keywords: 
yellow sweet clover (Melilotus officinalis (L.) Pall.)
herb
flavonoids
composition
content
References: 
  1. Zayko L. N. Atlas arealov i resursov lekarstvennyh rasteniy Rossii. M., 2008; 173–5.
  2. Talovina G.V. Rod Melilotus Mill. vo flore Rossii i sopredel`nyh stran. Diss. kand. biol. nauk. SPb, 2011; 124.
  3. Dikorastushhie lekarstvennye rasteniya Rossii: sbor, sushka, podgotovka syr`ya. Sbornik instrukciy.(Pod red. N.I.Sidel`nikova). M.: VILAR, 2015; 92–4.
  4. Nevidomova E.V., Nevidomova M.A., Nevidomov A.M. Cenopopulyaciya donnika lekarstvennogo (Melilotus officinalis L.) v antropogenno narushennyh associaciyah Nizhegorodskogo megapolisa. Voprosy biologicheskoy, medicinskoy i farmacetivcheskoy himii, 2015; 12: 18–23.
  5. Gosudarstvennyy reestr lekarstvennyh sredstv. M.: Minzdrav Rossii: Fond farmacevticheskoy informacii, 2004; 1277.
  6. Lokteva O.M. Farmakologicheskaya harakteristika e`kstrakta donnika lekarstvennogo. Diss. kand. biol. nauk. SPb., 1999; 130.
  7. Pantyuhina E.V. Razrabotka sostava, tehnologii mazi i medicinskogo karandasha antimikrobnogo deystviya s polie`tilenoksidnym e`kstraktom travy donnika lekarstvennogo. Diss. kand. farm. nauk. Pyatigorsk, 2008; 159.
  8. Harlampovich T.A. Fitohimicheskoe izuchenie i standartizaciya donnika lekarstvennogo travy, proizrastayushhey na territorii Altayskogo kraya. Avtoref. diss. kand. farm.nauk. Perm`, 2014, 22.
  9. Gosudarstvennaya farmakopeya SSSR XI izd., vyp. 2, M.: Medicina, 1990; 400.
  10. Saushkina A.S. i dr. Ispol`zovanie TSH dlya identifikacii biologicheski aktivnyh veshhestv v nekotoryh vidah lekarstvennogo syr`ya. Sorbcionnye i hromatograficheskie processy, 2001; 1 (5): 902–5.