DEVELOPMENT OF AN ENZYMATIC HYDROLYSIS PROCEDURE FOR THE ISOLATION OF DIMEDROL FROM HAIR SAMPLES

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DOI: https://doi.org/None
Issue: 
3
Year: 
2017

Yu.V. Slustovskaya, V.S. Kasheutova, O.Yu. Strelova, PhD Saint Petersburg State Chemical Pharmaceutical Academy; 14, Prof. Popov St., Saint Petersburg 197376, Russian Federation

Introduction. Traditional medicines can be used to produce narcotic and stupefying effects. So, for example, dimedrol intoxication has come to be often observed. Forensic chemical and chemotoxicological studies of urine and blood following 1–3 days usually yield negative results. Analysis of hair samples can detect toxicants over a much longer time period. Objective: to develop a procedure for detection of ground substances in the hair as an object of chemotoxicological studies (in case of dimedrol). Material and methods. Investigations were performed on outbred male white and black guinea-pigs, by using the reagent dimedrol substance. The dimedrol base in the extracts was quantified by gas chromatography-mass selective detection. Results. Alkaline hydrolysis and enzymatic hydrolysis may be used to extract the dimedrol base from hair. Enzymatic hydrolysis versus alkaline hydrolysis was ascertained to be more efficient. The enzymatic hydrolysis procedure was validated. Conclusion. A procedure for enzymatic hydrolysis by proteolytic enzymes has been developed, which can be used to disrupt the interaction of toxic substances with proteins in the hair chemotoxicological analysis.
Keywords: 
dimedrol
hair
alkaline hydrolysis
chemotoxicological analysis
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