DETERMINATION OF AUCUBIN IN THE GREATER PLANTAIN (PLANTAGO MAJOR L.) RAW MATERIALS AND PREPARATIONS

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DOI: https://doi.org/None
Issue: 
2
Year: 
2015

A.A. Zhogova (1,2); Professor I.A. Samylina (1), PhD; Professor K.I. Eller (2), PhD 1 -I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991 2 -Research Institute of Nutrition, Russian Academy of Sciences; 2/14, Ustyinsky Proezd, Moscow 109240

An HPLC procedure was developed to determine aucubin in the greater plantain (Plantago major) leaves. The relative error of single determination at 95% confidence interval was ±2.2%. The developed procedure could determine the amount of aucubin in greater plantain raw materials and preparations (infusion, tincture, juice). The content of aucubin in the samples of dried raw materials was 1.16 to 7.07 mg/g. Fairly high aucubin levels were determined in the greater plantain tincture (0.38 mg/ml) and juice (0.30-0.41 mg/ml). The findings suggest that the developed procedure should be used to standardize greater plantain raw materials, preparations, and supplements
Keywords: 
greater plantain
Plantago major L.
iridoids
aucubin
content
high-performance liquid chromatography
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