HPLC DETERMINATION OF NARCICCIN IN POT MARIGOLD (CALENDULA OFFICINALIS) FLOWERS

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DOI: https://doi.org/None
Issue: 
4
Year: 
2016

A.V. Kurkina (1), PhD; P.V. Afanasyeva (1); Professor V.A. Kurkin (1), PhD; Professor I.A. Platonov (2), PhD; L.V. Pavlova (2) 1 -Samara State Medical University; 89, Chapaevskaya St., Samara 443099, Russian Federation 2 -Academician S.P. Korolev Samara State Aerospace University (National Research University); 34, Moscovskoe Shosse, Samara 443086, Russian Federation

Background. The pharmacological properties of pot marigold (Calendula officinalis) preparations are due to their containing flavonoids, carotenoids, and saponins. The dominant substance of pot marigold flowers is the flavonoid narcissin (isorhamnetin-3-rutinoside) [11, 12]. This compound was first extracted from pot marigold by column chromatography. Objective: to develop a procedure for quantification of the flavonoid narcissin (isorhamnetin-3-rutinoside) in the pot marigold flowers (Flores Calendulae). Material and methods. The commercial samples of pot marigold flowers (OAO «Krasnogorskleksredstva, ZAO «Samaralektravy», Mid-Volga Branch, All-Russian Research Institute of Medicinal and Aromatic Plants) were the subjects of the investigation. Thin-layer (TLC) chromatography was performed using Sorbfil HPTLC-AF-A-UV plates in the chloroform-ethanol-water (26:16:3). UV spectra were recorded applying a Specord 40 spectrophotometer (Analytic Jena). High performance liquid chromatography (HPLC) analysis was carried out employing a Biotronic liquid chromatograph and a Phenomenex Luna C18(2) chromatographic column (250×4.6 mm; 5 μm). The standard samples of narcissin (isorhamnetin-3-O-rutinoside), rutin (quercetin-3-O-rutinoside), isoquercitrin (quercetin-3-O-β-D-glucopyranoside), and quercetin. Results. TLC analysis indicated that the predominant component of aqueous alcoholic extracts from pot marigold flowers was narcissin (isorhamnetin3-O-rutinoside), the chemical structure of which was close to that of rutin, comparable with the latter in mobility, and had close Rf and spectral characteristics. Investigations were conducted to develop a HPLC procedure to assay narcissin in the pot marigold flowers, which made it possible to calculate it in raw materials. Conclusion. The HPLC procedure was developed to quantify narcissin in the pot marigold flowers. The error of a single determination using the developed procedure with a 95% confidence probability was ±4.97%. The content of narcissin in the pot marigold flowers ranged from 0.68±0.02% to 0.97±0.03%.
Keywords: 
pot marigold
Calendula officinalis L.
flowers
flavonoids
narcissin
rutin
flavonoids
narcissin
rutin
High performance liquid chromatography
standardization
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