INVESTIGATION OF THE EFFECT OF A NEW 1,3-DIAZINON-4 DERIVATIVE DURING ITS LONG-TERM ADMINISTRATION ON LEUKOPOIESIS IN RATS

Download full text PDF
DOI: https://doi.org/None
Issue: 
6
Year: 
2016

A.V. Voronkov (1), MD; S.A. Luzhnova (2), PhD; S.A. Osychenko (1); G.N. Genatullina (2), PhD; Suda Billel (1) (Tunisia) 1 -Pyatigorsk Institute of Medicine and Pharmacy, Branch, Volgograd State Medical University; 11, Kalinin Pr., Pyatigorsk 357500, Russian Federation 2 -Leprosy Research Institute, Ministry of Health of Russia; 3. Nikolay Ostrovsky Passage, Astrakhan 414057, Russian Federation

Introduction. Diseases, such as leprosy and tuberculosis, require the long-term administration of antibacterial drugs. Dapsone is the primary drug for their treatment. However, it has a serious side effect on blood. A synthesized drug, a diazinon derivative, showed a high antimycobacterial activity and no toxic effect on blood counts in the experiment. Objective: to investigate the effect of PYATd1 versus Dapsone on leukopoiesis in rats. Materials and methods. An experiment was carried out in 60 Wistar rats of both sexes. Blood taken from the sublingual vein was stabilized with heparin. White blood cell counts were measured with a Mindray BC 2800vetaanalysis system used in veterinary hematology. Results. The long-term use of new 1,3-diazinon-4 derivative versus Dapsone was examined for its impact on white blood cell counts in the rats. The effects of the drugs on the counts of segmented neutrophils and eosinophils, young stab neutrophils and nuclear shift index were considered. Conclusion. No statistically significant changes in the complete blood count of the animals were observed during 90-day administration of the test compound, suggesting that it had no destructive effect on leukopoiesis in the rats.
Keywords: 
dapsone
diazinon derivative
nuclear shift index
neutrophils
eosinophils
stab neutrophils
References: 
  1. Balkaryova O.V., Fomin V.V., Kozlovskaya L.V. Agranulocytosis associated with the taking of cefoperazone by the patient with renal terminal kidney- failure. Therapeutic archive, 2005; 6: 76–78.
  2. Voronkov A.V., Luzhnova S. A., Osychenko S. A. et al. Appraisal of the effect of a new derivative of diazinonPYATd1 on some peripheral blood parameters of rats of both sexes.Vestnik of VolgGMU, 2015; 4 (56): 124–126.
  3. Kozinets G.I., Vysotsky V.V., Pogorelov V. M.et al. Blood and infection, Moscow: Triada-pharm, 2001; 456.
  4. Luzhnova S.A. Age aspects of the influence of Dapsone on the phagocytosis of white blood cells. Russian allergological journal, 2008; 1: 160–161.
  5. Luzhnova S.A., Gabitova N.M., Voronkov A.V. et al. Appraisal of antimicobacterial activity of new derivatives of diazinon. Fundamental research, 2015; 2 (11): 2377–2380.
  6. Luzhnova S.A., Samotrueva M.A., Yasenyavskaya A.L., Abdresheva R.J. Alpha-tocopherol as the correction of Dapsoneinduced changes in indices of leukogram. Fundamental science, 2013; 7: 580–583.
  7. Pivnik A.V., Tonkoglav V.N., Boyko D.V. Haptenagranulocytosis: treatment with hematopoietic growth factors. Clinical medicine, 2006; 3: 55–59.
  8. Tereshchenko I.P., Kashulina A.P. System role of neutrophilic granulocytes in the formation of features of development of the pathological process.Pathophisiology and experimental chemotherapy, 1993; 4: 56–60.
  9. Ghu G.I., Stiller M.G. Dapsone and sulfones in dermatology overview and update. Journal of the American Academy of dermatology, 2001; 45 (3): 420–434.
  10. Wozel G., Blasum C. Dapsone in dermatology and beyond. Arch. Dermatol. Res., 2014; 306 (2): 103–124.