DETERMINATION OF 2,4-DICHLOROPHENOXYACETIC ACID IN THE CHEMOTOXICOLOGICAL INVESTIGATION OF BIOLOGICAL MATERIAL

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DOI: https://doi.org/None
Issue: 
7
Year: 
2016

Professor V.K. Shormanov, PhD; T.F. Larina; M.V. Rymarova, PhD; Professor V.A. Korolev, PhD; A.I. Altukhova, PhD Kursk State Medical University; 3, K. Marx St., Kursk 305041, Russian Federation

Introduction. 2,4-dichlorophenoxyacetic acid is a substance that has herbicidal activity and also stimulates the growth of callus cultures and biosynthesis of their certain biologically active compounds. Objective: to develop sensitive methods for determining 2,4-dichlorophenoxyacetic acid in biological material. Material and methods. Model mixtures of 2,4-dichlorophenoxyacetic acid and liver tissue were used. Purification schemes were developed using liquid-liquid extraction and macrocolumn adsorption chromatography. Thin layer chromatography, gas chromatography–mass spectrometry, and reversed-phase high-performance liquid chromatography were used to identify and quantify the compound in question. Results. The insulating agent ethyl acetate was proposed for the extraction of 2,4-dichlorophenoxyacetic acid from biological material. Optimal extraction conditions were determined. Adsorption purification of 2,4-dichlorophenoxyacetic acid can be carried out in a silica gel L 40/100 μm column eluting with a mixture of the solvents hexane and diethyl ether (6:4). Conclusion. The developed procedure can identify and determine 86.64–88.26% of the initial amount of 2,4-dichlorophenoxyacetic acid in the model mixtures with liver tissues.
Keywords: 
2
4-dichlorophenoxyacetic acid
biological material
extraction
chromatography
identification and determination
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