Standardization of the composition of growth substrates for obtaining an antifungal antibiotic

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DOI: https://doi.org/10.29296/25419218-2019-05-04
Issue: 
5
Year: 
2019

E.P. Yakovleva, V.A. Kolodyaznaya, O.V. Topkova Saint Petersburg State Chemopharmaceutical University, 8, Prof. Popov St., Saint Petersburg 197376, Russian Federation

Introduction. Natural raw materials with their unstable chemical composition are used as a source of carbohydrates in the biotechnology industry. Synthetic sugars, aryl-substituted xylose derivatives possessing antimicrobial activity do not have this disadvantage. Objective: to investigate synthetic sugars as an additional source of carbohydrates in the composition of a growth medium, as well as a means of protecting the cultivation process of a producer from possible contamination. Material and methods. The investigation object was an antibiotic imbricin producer by the actinomycete Streptomyces imbricatus strain 0112/90, as well as synthetic sugars. Results. It was shown that synthetic sugars could not serve as an additional source of carbohydrate nutrition, but have an antimicrobial effect. Conclusion. The synthetic saccharide (3,4-dinitrophenyl-β-D-xyloxylopyranoside) at a 0.1% concentration can be recommended for addition to the composition of a growth medium as a means of protection against possible contamination at the fermentation stage.
Keywords: 
cultivation process
aryl-substituted saccharides
antimicrobial activity
contamination
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