Methods for determining mercury in biological objects

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DOI: https://doi.org/10.29296/25419218-2020-03-01
Issue: 
3
Year: 
2020

N.O. Kim, E.A. Ivanovskaya Novosibirsk Medical State University; 52, Krasnyi Prospect, Novosibirsk 63001, Russian Federation

To ensure the quality of a pharmaceutical substance and ready-made drugs during development and optimization processes and a process change, it is necessary to carefully control one of its most important indices – impurities. Mercury is a heavy metal that can enter the body with seafood and hydrobionts, so the substances derived from marine biocenosis products may contain mercury as an impurity. It is important not only to carry out a qualitative analysis, but also to find an effective quantitative analysis of mercury-containing drugs at all stages of a production process. The aim of this investigation was to review the literature on methods for determining mercury in biological objects. The paper presents data on existing up-to-date methods to determine mercury, as one of the toxic elements that can accumulate and is contained in organic substances, as well as in pharmaceutical production sources, including those based on protamine. The review of the literature has shown that pharmacopoeial (dithizone extraction/photocolorimetry), spectrophotometric (including flameless or cold vapor atomic absorption spectrometry), chromatographic (mainly high performance liquid chromatography) and electrochemical (including voltammetric analysis) techniques are used to measure mercury as an impurity in the sources of pharmaceutical production.
Keywords: 
mercury
determination methods
spectrometry
chromatography
voltamperometry
protamine
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