Investigation of the amino acid composition of a nootropic herbal tea

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DOI: https://doi.org/10.29296/25419218-2020-03-03
Issue: 
3
Year: 
2020

E.A. Dorovskikh, V.A. Ermakova, T.Yu. Kovaleva I.M. Sechenov First Moscow State Medical University (Sechenov University); 8, Trubetskaya St., Build. 2, Moscow 119048, Russian Federation

Introduction. The number of patients who need nootropic drugs is constantly growing both in Russia and around the world. In this connection, it is relevant to design effective and safe medicines for the treatment and prevention of these diseases. Chemical screening of a nootropic herbal tea and its individual components has shown that they contain nitrogenous compounds, including amino acids. Taking into account the important role of amino acids in the functioning of the nervous system, a more detailed study of this group of biologically active compounds is of scientific and practical interest. Objective: to investigate the qualitative and quantitative composition of free and bound amino acids in the nootropic herbal tea. Material and methods. The investigation object was an air-dry ground nootropic herbal tea. A detailed qualitative and quantitative analysis was carried out by TLC and HPLC with pre-column derivatization of samples and separation of amino acids by reversed-phase chromatography on a fluorometric detector, by applying the Waters AccQ Tag method. Results. The amino acid composition of a nootropic herbal tea was represented by 16 amino acids in free and bound forms, 7 of which were essential. The total content of free and bound amino acids was 1.165 and 3.216%, respectively. Conclusion. The investigation of biologically active substances in the nootropic herbal tea provided data on the composition and quantitative content of amino acids.
Keywords: 
nootropic drugs
herbal tea
amino acids
Waters AccQ Tag method
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