Some aspects of the cerebroprotective effects of extracts from the herb of representatives of the genus Prunella

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DOI: https://doi.org/10.29296/25419218-2020-05-08
Issue: 
5
Year: 
2020

A.A. Shamilov(1), D.I. Pozdnyakov(1), V.N. Bubenchikova(2), M.V. Chernikov(1), E.R. Garsia(1), 1-Pyatigorsk Medical and Pharmaceutical Institute, Branch, Volgograd State Medical University, 11, Kalinin Pr., Pyatigorsk 357532, Russian Federation; 2-Kursk State Medical University; 3, K. Marx St., Kursk 305041, Russian Federation

Introduction. Cerebral protection mediated by the administration of herbal agents is a promising approach to the adjuvant therapy of ischemic stroke. The genus Prunella L. (self-heal) has 15 species that are characterized by a broad spectrum of pharmacological activity. Objective: to experimentally evaluate the cerebroprotective effect of extracts from the herb of three self-heal species. Material and methods. The investigation objects were aqueous and aqueous-alcoholic extracts from the herb of big-flowered self-heal (Prunella grandiflora), cut-leaved self-heal (Prunella laciniata), and common self-heal (Prunella vulgaris) gathered in the North Caucasus. Their cerebroprotective effect was evaluated on a rat model of permanent focal ischemia, by determining changes in the rate of cerebral blood flow, in the size of a brain necrotic area, and in the serum concentration of lactic acid. Results. The use of dry residues obtained with 70% and 40% ethyl alcohol from the herb of big-flowered, cut-leaved, and common self-heals assisted in decreasing serum lactate levels in the animals, restoring cerebral blood flow rate, and reducing the cerebral necrotic area to a comparable extent with the standardized extract of ginkgo biloba (EGB 671). Conclusion. Further investigations of extracts from the herb of three self-heal species were shown to be promising in identifying the possible mechanisms of cerebroprotective action.
Keywords: 
cerebral ischemia
cerebroprotective effect
big-flowered self-heal (Prunella grandiflora L.)
cut-leaved self-heal (Prunella laciniata L.)
and common self-heal (Prunella vulgaris L.)
References: 
  1. Neuhaus A.A., Couch Y., Hadley G., Buchan A.M. Neuroprotection in stroke: the importance of collaboration and reproducibility. Brain. 2017; 140 (8): 2079–92. DOI: 10.1093/brain/awx126
  2. Bennion D.M., Steckelings U.M., Sumners C. Neuroprotection via AT2 receptor agonists in ischemic stroke. Clin. Sci. (Lond). 2018; 132 (10): 1055–67. DOI: 10.1042/CS20171549
  3. Yamashita T., Abe K. Recent Progress in Therapeutic Strategies for Ischemic Stroke. Cell Transplant., 2016; 25 (5): 893–8. DOI: 10.3727/096368916X690548
  4. Aruoma O.I., Bahorun T., Jen L.S. Neuroprotection by bioactive components in medicinal and food plant extracts. Mutat. Res. 2003; 544 (2–3): 203–15. DOI: 10.1016/j.mrrev.2003.06.017
  5. Kuboyama T., Tohda C., Komatsu K. Effects of Ashwagandha (roots of Withania somnifera) on neurodegenerative diseases. Biol. Pharm. Bull. 2014; 37 (6): 892–7. DOI: 10.1248/bpb.b14-00022
  6. Wang S.J., Wang X.H., Dai Y.Y. et al. Prunella vulgaris: A Comprehensive Review of Chemical Constituents, Pharmacological Effects and Clinical Applications. Curr. Pharm. Des. 2019; 25 (3): 359–69. DOI:10.2174/1381612825666190313121608
  7. Rastitel'nye resursy Rossii: Dikorastuschie tsvetkovye rastenija, ih komponentnyj sostav i biologicheskaja aktivnost'. Tom 4. Semejstvo Caprifoliaceae – Lobeliaceae (pod red. P.D. Sokolova). SPb., M.: Tovarischestvo nauchnyh izdanij KMK, 2011; 236–9. [Plant resources of Russia. Flowering wild plants, their phytochemical constituents and biological activities. Family Caprifoliaceae – Lobeliaceae. Tom 4. (by ed. P.D.Sokolov). Saint Peterburg, Moscow: Tovarishestvo nauchnikh izdaniy KMK, 2011; 236–9 (in Russian)]
  8. Bai Y., Xia B., Xie W. et al. Phytochemistry and pharmacological activities of the genus Prunella. Food. Chem. 2016; 204: 483–96. DOI: 10.1016/j.foodchem.2016.02.047
  9. Shamilov A.A. Rastenija roda Prunella: Himicheskij sostav, vidy farmakologicheskogo dejstvija. Vestnik Voronezhskogo gosudarstvennogo universiteta. Serija: Himija. Biologija. Farmatsija, 2017; 4: 152–60. [Shamilov A.A. Plants of the genus Prunella: chemical composition, species pharmacologicalaction. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya. 2017; 4: 152–60 (in Russian)].
  10. Pozdnyakov D.I., Voronkov A.V., Zolotych D.S., Arlt A.V. Antioxydant and endotheliotropic properties 4-hydroxy-3,5-di-tret-butyl cinnamic acid under conditions of experimental brain ischemia. J. of Young Pharmacists. 2018; 10 (2): 193–6.
  11. Voronkov A.V., Pozdnyakov D.I. Endothelotropic activity of 4-hydroxy-3,5-di-tret-butylcinnamic acid in the conditions of experimental cerebral is chemia. Research Results in Pharmacology. 2018; 4 (2): 1–10.
  12. Aloglu A. K., Harrington P. D. B., Sahin S., Demir C. Prediction of total antioxidant activity of Prunella L. species by automatic partial least square regression applied to 2-way liquid chromatographic UV spectral images. Talanta. 2016; 161: 503–10. DOI: 10.1016/j.talanta.2016.09.014