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Features of the body distribution of ceftriaxone in warm-blooded animals


A.A. Bez’yazychnaya, V.K. Shormanov, L.E. Siplivaya, B.M. Tagirmirzoev, M.V. Rymarova, I.M. Zhukov Kursk State Medical University, 3, Karl Marx St., Kursk 305004, Russian Federation

Introduction. Ceftriaxone is a yellowish-white crystalline powder, is hygroscopic and readily soluble in water. It is a third-generation antibiotic with a broad spectrum of action. Objective: to study of the body distribution of ceftriaxone in warm-blooded animals when it is given at lethal doses. Material and methods. The distribution of the substance in free form or included in the erythrocyte carriers was studied in the groups of healthy mice and those with simulated toxic kidney damage caused by HgCl2. Isolation was carried out with an acetone-water mixture of 5:5 at a mass ratio of the insulating mixture and the biological object (2:1). The investigators used thin-layer chromatography, ultraviolet spectrophotometry, and high performance liquid chromatography for the identification of ceftriaxone and UV spectrophotometry for its quantitative determination. Results. Ceftriaxone was found in large quantities in the visceral organs and blood of poisonous organisms. Ceftriaxone intravenously injected in free form to healthy animals was present in the highest amounts in the bladder with urine (2718.93 mg/100 g), whereas it was also seen in the blood (523.02 mg/100 mg) and urinary bladder (571.10 mg/100 mg) of animals with simulated toxic kidney damage caused by HgCl2. When ceftriaxone included into the erythrocyte carriers was intravenously injected to the healthy mice, the maximum concentration of the drug was observed in the blood (937.57 mg/100 g) and urinary bladder (1184.59 mg/100 g). When erythrocyte carriers with ceftriaxone were administered to the group of mice with simulated renal toxicity induced by HgCl2, there were changes in the distribution of the test antibiotic. The peak concentration was observed in the kidneys (668.61 mg/100 g) and blood (663.41 mg/100 g). Conclusion. The results of the investigations make it possible to imagine differences in the nature of ceftriaxone distribution in the warm-blooded animals in health and in simulated renal disease when the antibiotic was intravenously injected as a substance or part of erythrocyte carriers.

erythrocyte carriers
mercury dichloride
identification and determination

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