The development of technology for therapeutic and prophylactic herbal toothpaste and its standardization


N.R. Varina, V.A. Kurkin, E.V. Avdeeva, S.V. Pervushkin, L.D. Klimova, V.V. Stenyaeva, N.I. Zimenkina Samara State Medical University, 89, Chapaevskaya St., Samara 443099, Russian Federation

Introduction. The relevance of designing dosage forms for dental practice, including therapeutic and prophylactic toothpastes, is due to several factors. First of all, due to the fact that oral hygiene is one of the most important and most widespread preventive measures for oral infectious and inflammatory diseases. This in turn assists in preventing the diseases and conditions associated with chronic infection foci in the oral cavity. Therefore, hygienic care of the teeth and oral cavity not only prevents dental diseases, but also reduces the infectious load on the body as a whole. Objective: to develop a technology for therapeutic and prophylactic Dentos phytocomposition-based toothpaste, to investigate its characteristics and to work out some indicators of its quality, and to analyze the findings. Material and methods. The investigation objects were a Dentos herbal remedy based on five types of medicinal plant materials, as well as samples of various toothpaste compositions. The investigators used technological methods for preparing the toothpaste, analytical methods for assessing the quality indicators of the designed composition: methods for determining homogeneity, plasticity, pH value, the height of the foam column, and foam stability; and phytochemical methods for pharmacopoeial assays (qualitative test tube reactions, TLC, and UV spectrophotometry). Results. The authors provided evidence that it was expedient to design therapeutic and prophylactic herbal toothpastes made in Russia. The compositions of toothpastes based on sodium-CMC were obtained, by using the following abrasives: precipitated calcium carbonate, aerosil, and also Dentos tincture in various ratios. The composition that was optimal in terms of ensuring and preserving the organoleptic characteristics of the toothpaste was selected. The step-by-step technological scheme for the toothpaste was substantiated. The indicators characterizing its quality were identified. For a qualitative analysis, the investigators proposed to use the nature of the UV spectrum and a reaction with flavonoids and tannins. They also devised a method for the quantitative spectrophotometric determination of the amount of phenylpropanoids calculated with reference to caffeic acid. Conclusion. The composition of therapeutic and prophylactic toothpaste based on the Dentos herbal remedy was designed and its technology developed. The organoleptic and technological characteristics of the toothpaste were studied. The investigators proposed its quality indicators, such as appearance, color, odor, taste, mass fraction of water, hydrogen index (pH), foam height, foam stability, qualitative and quantitative determination of biologically active compounds.

therapeutic and prophylactic toothpaste
Dentos herbal remedy
antimicrobial and anti-inflammatory effect
toothpaste technology
biologically active compounds

  1. Averyanov C.V., Galiullina E.F., Shakirov D.F. Modern aspects of therapeutic and prophylactic therapy of inflammatory periodontal disease. Uspekhi sovremennoy nauki i obrazovaniya. 2017; 4 (3): 71–6 (In Russian)
  2. Orekhova L.Yu., Kudryavtseva T.V., Burlakova Yu.S. Systems for local drug delivery in periodontology. Parodontologiya. 2016; 21 (1): 34–9 (In Russian)
  3. Tsarev V.N., Nikolaev E.N., Ippolitov E.V. Periodontopathogenic bacteria – the main factor in the occurrence and development of periodontitis. Zhurnal mikrobiologii, epidemiologii i immunobiologii. 2017; 5: 101–12 (In Russian)
  4. Ritam S. Naiktari, Pratima Gaonkar, Abhijit N. Gurav, Sujeet V. Khiste. A randomized clinical trial to evaluate and compare the efficacy of triphala mouthwash with 0.2% chlorhexidine in hospitalized patients with periodontal diseases. J. Periodontal. Implant. Sci. 2014; 44: 134–40. DOI:10.5051/jpis.2014.44.3.134
  5. Robert P., Allakera C.W., Douglasb I. Novel anti-microbial therapies for dental plaque-related diseases. International Journal of Antimicrobial Agents. 2009; 33: 8–13. DOI: 10.1016/j.ijantimicag.2008.07.014
  6. Sälzer S., Rosema N.A., Martin E.C. et al. The effectiveness of dentifrices without and with sodium lauryl sulfate on plaque, gingivitis and gingival abrasion: a randomized clinical trial. Clin. Oral. Investig. 2016; 20 (3): 443–50.
  7. Stepanov G.V., Ayupova I.O., Ulyanova L.G. Studying the results of laboratory studies of the oral fluid and blood serum of patients in the retention period of orthodontic treatment. Nauka i innovatsii v meditsine. 2016; 2 (2): 29–32 (In Russian)
  8. Urazova R.Z., Musina L.T., Egorova A.B. Comparative analysis of antiseptic toothpastes. Prakticheskaya meditsina. 2009; 1: 84–6 (In Russian)
  9. Sarap L.R., Zhilenko O.G., Podzorova E.A., Lesnykh I.V. Therapeutic and prophylactic effectiveness of toothpastes based on natural ingredients in patients with inflammatory periodontal diseases. Klinicheskaya stomatologiya. 2009; 3: 40–2 (In Russian)
  10. Sirak S.V. The study of anti-inflammatory and regenerative properties of a dental gel based on plant components, glucosamine hydrochloride and dimexide in the experiment. Parodontologiya. 2010; 1 (54): 46–50 (In Russian)
  11. Hotwani K., Baliga S., Sharma K. Phytodentistry: use of medicinal plants. J. Complement. Integr. Med. 2014; 11 (4): 233–51.
  12. Abdurakhmanova S.A., Runova G.S. A review of modern herbal remedies used in the treatment of inflammatory periodontal diseases. Rossiyskaya stomatologiya. 2018; 11 (4): 37–41 (In Russian)
  13. Borisova E.G., Pototskaya A.V. Experience with the use of periodontal drugs for the treatment and prevention of periodontal disease. The Journal of scientific articles «Health and Education Millennium». 2017; 9 (7): 17–20 (In Russian)
  14. Gontarev S.N., Gontareva I.S., Nikishaev A.V. The use of herbal remedies in pediatric dentistry. Nauchnyye rezul'taty biomeditsinskikh issledovaniy. 2016; 2 (2): 17–21 (In Russian)
  15. Goncharova E.I. Drugs of medicinal plants in the treatment of diseases of the oral mucosa. Rossiyskiy stomatologicheskiy zhurnal. 2015; 19 (4): 55–7 (In Russian)
  16. Lapina A.S., Varina N.R., Kurkin V.A. et al. HPLC analysis of the terpenoid complex of rod eucalyptus (Eucalyptus viminalis Labill.). Aspirantskiy vestnik Povolzh'ya. 2018; 1–2: 17–21 (In Russian)
  17. Slobodníková L., Fialová S., Rendeková K. et al. Antibiofilm activity of plant polyphenols. Molecules. 2016; 21 (12).
  18. Vieira D.R., Amaral F.M., Maciel M.C. et al. Plant species used in dental diseases: ethnopharmacology aspects and antimicrobial activity evaluation. J. Ethnopharmacol. 2014; 155 (3): 1441–9.