An investigation of the composition of essential oil in the woody greenery of Scotch pine (Pinus sylvestris)


A.M. Sumenkova, D.K. Gulyaev, V.D. Belonogova, P.S. Mashchenko Perm State Pharmaceutical Academy, 2, Polevaya St., Perm 614990, Russian Federation

Introduction. Scotсh pine (Pinus sylvestris L.) is a widespread woody plant in the Russian Federation. The pine woody greenery consisting of needles and branches are the main raw material to obtain essential oil. The elemental composition of the needles and branches can differ, which will affect the pharmacological activity of the resulting essential oil. Objective: to investigate the composition of essential oil in the Scotch pine needles and branches and to identify main components. Material and methods. The investigation objects were samples of Scotch pine greenery collected in the grass pine forest in the Ilyinsky District of the Perm Territory. Essential oil was obtained by hydrodistillation of pine needles and branches using the Ginsberg apparatus. Chromatography-mass spectrometric analysis was carried out to determine the elemental composition of the essential oil. Results. The composition of essential oil in the Scotch pine needles and branches was established to differ in qualitative and quantitative terms. There were 29 and 18 components in the essential oil of Scotch pine needles and branches, respectively. The main components of essential oil were ∆3-karen and limonene in the pine needles and aromadendrene and ∆3-karen in the branches. Conclusion. A change in the ratio of needles to branches in the raw material to obtain essential oil can affect the properties of the final product, since the composition of the essential oil differs significantly in the Scotch pine needles and branches.

Scotch pine
Pinus sylvestris L.
essential oil
elemental composition

  1. Lomatkin S.A., Skakovskij E.G., Mehanikova E.G. i dr. Sezonnaja dinamika terpenovyh uglevodorodov efirnogo masla sosny obyknovennoj (Pinus sylvestris L.). Trudy BGTU. 2019; 1: 17–24. [Lomatkin S.A., Skakovskiy Ye.G., Mekhanikova Ye.G. at al. Seasonal dynamics of terpene hydrocarbons of common pine essential oil (Pinus sylvestris L.). Trudy BGTU. 2019; 1: 17–24 (in Russian)]
  2. Sotnikova O.V, Stepen' R.A. Efirnye masla sosny kak indikator zagrjaznenija sredy. Himija rastitel'nogo syr'ja. 2001; 1: 79–84. [Sotnikova O.V, Stepen R.A. Pine essential oils as an indicator of environmental pollution. Khimiya rastitelnogo syrya. 2001; 1: 79–84 (in Russian)]
  3. Plemenkov V.V., Tevs O.A. Mediko-biologicheskie svojstva i perspektivy terpenoidov (izoprenoidov). Himija rastitel'nogo syr'ja. 2014; 4: 5–20. [Plemenkov V.V., Tevs O.A. Biomedical properties and prospects of terpenoids (isoprenoids). Khimiya rastitelnogo syrya. 2014; 4: 5–20 (in Russian)]. DOI: 10.14258/jcprm.201404225.
  4. Savel'eva E.E., Efremov A.A. Antioksidantnaja aktivnost' efirnyh masel nekotoryh dikorastuschih drevesnyh rastenij Sibiri. Vestnik Krasnojarskogo gosudarstvennogo agrarnogo universiteta. 2017; 2: 141–7. [Savelyeva Ye.Ye., Yefremov A.A. Antioxidant activity of essential oils of some wild woody plants of Siberia. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta. 2017; 2: 141–7 (in Russian)]
  5. Süntar I., Tumen I., Ustün O. at al. Appraisal on the wound healing and anti-inflammatory activities of the essential oils obtained from the cones and needles of Pinus species by in vivo and in vitro experimental models. J. Ethnopharmacol. 2012; 139 (2): 533–40. DOI: 10.1016/j.jep.2011.11.045
  6. Fidyt K., Fiedorowicz A., Strządała L. at al. β-caryophyllene and β-caryophyllene oxide-natural compounds of anticancer and analgesic properties. Cancer Med. J. 2016; 5 (10): 3007–17. DOI: 10.1002/cam4.816
  7. Gosudarstvennaja farmakopeja Rossijskoj Federatsii. XIV izdanie. Tom 2. OFS. «Opredelenie soderzhanija efirnogo masla v lekarstvennom rastitel'nom syr'e i lekarstvennyh rastitel'nyh preparatah». [Elektronnyj resurs]. Rezhim dostupa: [The State Pharmacopoeia of the Russian Federation, XIV-ed. OFS. «Determination of the content of essential oil in herbal raw materials and herbal medicines». [Electronic resource]. Access mode: (in Russian)].
  8. Crowell P.L., Elson C.E., Bailey H.H. et al. Human metabolism of the experimental cancer therapeutic agentd-limonene. Cancer Chemother. Pharmacol. 1994; 35: 3–31.
  9. Rabi T., Bishayee A. Terpenoids and breast cancer chemoprevention. Breast Cancer Res. Treat. 2009; 115 (2): 223–239. DOI: 10.1007/s10549-008-0118-y.
  10. Soundharrajan I., Kim D.H., Srisesharam S. et al. R-Limonene Enhances Differentiation and 2-Deoxy-D-Glucose Uptake in 3T3-L1 Preadipocytes by Activating the Akt Signaling Pathway. Evid. Based Complement Alternat Med. 2018; 5: 1–10. DOI: 10.1155/2018/4573254
  11. Tan X.C., Chua K.H., Ravishankar Ram M. et al. Monoterpenes: Novel insights into their biological effects and roles on glucose uptake and lipid metabolism in 3T3-L1 adipocytes. Food Chem. X. 2016; 196: 242–50. DOI: 10.1016/j.foodchem.2015.09.042
  12. Murali R., Karthikeyan A., Saravanan R. Protective effects of D-limonene on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rats. Basic Clin. Pharmacol. Toxicol. 2013; 112 (3): 175–81. DOI: 10.1111/bcpt.12010
  13. Yang L., Liu J., Li Y. et al. Bornyl acetate suppresses ox-LDL-induced attachment of THP-1 monocytes to endothelial cells. Biomed. Pharmacother. 2018; 103: 234–9. DOI: 10.1016/j.biopha.2018.03.152
  14. Li J., Wang S.X. Synergistic enhancement of the antitumor activity of 5-fluorouracil by bornyl acetate in SGC-7901 human gastric cancer cells and the determination of the underlying mechanism of action. J. BUON. 2016; 21 (1): 108–17.
  15. Matejić J.S., Stojanović-Radić Z.Z., Ristić M.S. at al. Chemical characterization, in vitro biological activity of essential oils and extracts of three Eryngium L. species and molecular docking of selected major compounds. J. Food Sci. Technol. 2018; 55 (8): 2910–25. DOI: 10.1007/s13197-018-3209-8