Development of a bioanalytical procedure for determination of a new antioxidant by a voltammetric method

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DOI: https://doi.org/10.29296/25419218-2021-08-02
Issue: 
8
Year: 
2021

T.G. Shinko(1), S.V. Terentyeva(1), E.A. Ivanovskaya(1), O.I. Prosenko(2) 1-Novosibirsk State Medical University, 52, Krasnyi Prospect, Novosibirsk 630091, Russian Federation; 2-Novosibirsk State Pedagogical University, 28, Vilyuiskaya St., Novosibirsk 630126, Russian Federation

Introduction. The new promising sulfur-containing phenolic antioxidant dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide has been developed at the Department of Chemistry, Novosibirsk State Pedagogical University. The antioxidant activity of this compound has been shown in studies using various models. It has also been found to be able to have hepatoprotective and anti-inflammatory activities, hemorheological, antiaggregatory, antiplatelet, and pronounced protective effects in cerebral ischemia. Objective: to develop a bioanalytical procedure for estimating the content of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide in biological material for further pharmacokinetic studies Material and methods. Differential pulse voltammetry was chosen to develop this procedure. The determination was carried out on a working mercury-film (amalgam) electrode using a silver chloride electrode as a reference electrode. The background electrolyte was a mixture of 95% ethanol and 2M sodium hydroxide solution (4:6). Results. The electrochemical behavior of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide was investigated and conditions for its rat serum quantification were selected. The developed procedure was validated. This procedure could establish the pharmacokinetic parameters of dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide in the rats after its single intragastric administration at a dose of 500 mg/kg.Conclusion. The developed procedure for the voltammetric determination of rat serum dodecyl (3,5-dimethyl-4-hydroxybenzyl) sulfide can be used to identify its pharmacokinetic parameters during preclinical studies
Keywords: 
dodecyl (3
5-dimethyl-4-hydroxybenzyl) sulfide
voltammetry
validation
pharmacokinetics
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