DEVELOPMENT OF APPROACHES TO STANDARDIZATION OF ACHILLEA MILLEFOLIUM HERBS

Introduction. According to Pharmacopoeial monograph 2.5.0101.18 “Achilleae millefolii herba” it’s indicated that in the stage of isolation from the raw materials acid hydrolysis using a mixture of ethyl alcohol 96% and concentrated hydrochloric acid 1% with following determination of the total flavonoids calculated on luteolin is necessary. Taking into account the fact that Yarrow (Achillea millefolium L.) contains the flavonoids, including 7-O-glucosides of luteolin (cynaroside) and apigenin (cosmosiin) which are hard to extract with acid hydrolysis, research shows an actual in terms of improving the methods of the quantitative determination of total flavonoids in the plant raw material. Objective: to develop a method of quantitative determination of the total flavonoids in the herb of Achillea millefolium L. Material and methods. Material of studies was served herb of Achillea millefolium L., harvested in 2020 and 2021, and also industrial sample from JSC “Krasnogorskleksredstva” Differential spectrophotometry was used as a method of investigation, held according to Pharmacopoeial monograph 1.2.1.1.0003.15 “Spectrophotometry in ultraviolet and visible spectrum”. Spectral characteristics of water-ethanolic extraction was marked on spectrophotometer “Specord 40” (Analytik Jena AG, Germany) in a cuvette with layer thickness 10 mm. Results. There was developed the methods for the quantitative determination of the total flavonoids in herb of Achillea millefolium L. We used the differential spectrophotometry method with the state standard sample of cynaroside at an analytical wavelength of 400 nm. The content of total flavonoids in the plant raw material is about 0,41±0,02 to 0,74±0,03% (calculated on cynaroside). The error of a single determination with a confidence level of 95% is ±6.70%. Conclusion. In the result of the studies the expedience of the quality evaluation of Achillea millefolium herbs in substance of the total flavonoids calculated on cynaroside has been substantiated, and with saving a numerical statistic “The total flavonoids in the plant raw material should be not less 0,4%” according to Pharmacopoeial monograph 2.5.0101.18 “Achilleae millefolii herba”