The study of the morphological and anatomical structure of the Lathyrus tuberosus

DOI: https://doi.org/10.29296/25419218-2022-05-01
Issue: 
5
Year: 
2022

K.R. Bubenchikova(1), I.V. Paskar(2)
1-Kursk State Medical University, St. K. Marx, h. 3, Kursk, Kursk region, 305041, Russian Federation;
2-«Farmoborona» Test Center Ltd, St., Gagarin 46A, Korolyov, Moscow Region, 141074, Russian Federation

Introduction. Tuberosus chin (Lathyrus tuberosus L.) is a plant widely represented in the chernozem zone of Russia. It contains various groups of biologically active substances: flavonoids, polysaccharides, terpenoids, tannins, amino acids. The plant is used in folk medicine. There are no data on the morphological and microscopic examination of the raw materials of the Lathyrus tuberosus. Objective: establishing indicators of the authenticity of the of Lathyrus tuberosus herb, including studies of morphological and microdiagnostic characteristics of raw materials. Material and methods. As an object of study was used the aerial part of the Lathyrus tuberosus, both fresh and dried, collected in the Kursk region in 2019–2021. Macroscopic studies were carried out with the naked eye and using a binocular loupe. Microscopic signs of various organs of the Lathyrus tuberosus were analyzed by preparing temporary preparations, using a «Micromed C1 LED» microscope with a digital attachment. Results. Morphological and microdiagnostic features of Lathyrus tuberosus herb, including stem, leaf, calyx and corolla, have been established. The main microdiagnostic features are: the pedicle is of a bundle type of structure, the vascular bundles are closed, collateral. Anomocytic stomata. The pubescence includes three types of hairs: three-celled with thick-walled cells, their basal part consists of two small prosenchymal cells, the terminal cell is long with a pointed apex (leaflets of a compound leaf); simple thin-walled one-two-celled hairs with a pointed apex and simple thin-walled two-three-celled hairs with an elongated terminal cell and a rounded apex with granular contents (calyx). The edge of the corolla has thin-walled papillary outgrowths. Prismatic crystals are located along the veins of the leaflets of the compound leaf and the calyx. Conclusion. The investigation has provided new data on the morphological and anatomical structure of Lathyrus tuberosus herb.
Keywords: 
Lathyrus tuberosus L.
herb
morphological signs
microdiagnostic signs.
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