Development of a method for assessing drugs antiviral activity

DOI: https://doi.org/10.29296/25419218-2022-05-08
Issue: 
5
Year: 
2022

B.A. Imamaliev, R.R. Shokodirov
Scientific Center «Med Standart LLC», Karasaray street 343, Almazar district, Tashkent, 100049, Republic of Uzbekistan

Introduction: to date, for the experimental evaluation of the antiviral activity of drugs, a number of in vitro and in vivo methods based on the use of virus strains is used. However, the disadvantage of all these methods is that it does not always happen that one or another virus strain is available to the experimenter, which makes it difficult to assess the antiviral activity of the drug. Purpose of the study: development of a method for evaluating the antiviral evaluation of drugs, without the use of virus strains. Material and methods: the authors suggest that antiviral preparations will have a protective effect on erythrocytes under conditions of osmotic hemolysis, as well as have a protective effect on the cell under conditions of a viral attack of the cell. It should be noted that the literature does not provide information on the protective properties of antiviral agents under conditions of osmotic hemolysis, which makes this development unique. In the development, a method was used based on the assessment of the osmotic activity of erythrocytes for a new purpose. Results: according to the results of the experiment, it was found that reference antiviral preparations have a protective effect on blood cells under conditions of osmotic hemolysis. Conclusion: a new method has been developed based on the ability of an antiviral drug to have a protective effect on erythrocytes under conditions of osmotic hemolysis, which can be recommended for experimental study of the antiviral activity of medicinal and pharmacological preparations with antiviral activity.
Keywords: 
antiviral activity
method
osmotic hemolysis
erythrocytes.
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