Investigation of flavonoids of the herb alfalfa sickle (Medicago falcata L.)

DOI: https://doi.org/10.29296/25419218-2022-08-02
Issue: 
8
Year: 
2022

N.N. Shvets, V.N. Bubenchikova
FSBEI HE KSMU Ministry of Health of the St. K. Marx, h. 3, Kursk, Kursk region, 305041, Russian Federation

Introduction. Crescent alfalfa (Medicago falcata L.) is widespread in the regions of central Russia, it is a fodder and honey plant. The plant contains various classes of biologically active substances, among which flavonoids, isoflavonoids are more valuable, because. have different pharmacological efficacy, such as anti-inflammatory, anticarcinogenic, antimicrobial, antioxidant. In this regard, the identification and qualitative determination of individual flavonoids of the herb sickle alfalfa by various methods is relevant. Objective: study of the flavonoid compounds of the alfalfa herb by known qualitative reactions, paper chromatography and ultra performance liquid chromatography/MS. Material and methods. The flavonoid composition of sickle alfalfa was studied using qualitative reactions, paper chromatography, ultra performance liquid chromatography/MS on a Waters Acquility chromatograph with a UV diode array detector and a TQD tandem quadrupole MS detector (Waters) in the gradient elution mode. Flavonoid compounds were extracted with 70% ethyl alcohol. Results. The flavonoid composition of the crescent alfalfa herb is represented by derivatives of quercetin, myricetin, apigenin, mainly their methoxylated forms. A total of 10 compounds were identified, including 8 flavonols, which were divided into quercetin derivatives (rutin, 4',7-O-Dimethylquercetin-3-O-di-methylglucoside, 4',7-O-dimethylquercetin-3-O-methylglucoside) , the rest are classified as myricetin derivatives. Two compounds are classified as flavones (apigenin-7-O-methylglucoside, apigenin-7-O-acetylglucosyl, glucosylglucoside). Conclusion. 10 flavonoid compounds, represented mainly by methoxylated compounds, were identified in the herb alfalfa sickle, and therefore it can serve as a potential source of these compounds.

Keywords: 
herba
sickle alfalfa
Medicago falcata L.
flavonoids
ultra performance liquid chromatography with photodiode array and tandem quadrupole mass selective detection.

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