Investigation of flavonoids of the herb alfalfa sickle (Medicago falcata L.)
Issue:
8
Year:
2022
Introduction. Crescent alfalfa (Medicago falcata L.) is widespread in the regions of central Russia, it is a fodder and honey plant. The plant contains various classes of biologically active substances, among which flavonoids, isoflavonoids are more valuable, because. have different pharmacological efficacy, such as anti-inflammatory, anticarcinogenic, antimicrobial, antioxidant.
In this regard, the identification and qualitative determination of individual flavonoids of the herb sickle alfalfa by various methods is relevant.
Objective: study of the flavonoid compounds of the alfalfa herb by known qualitative reactions, paper chromatography and ultra performance liquid chromatography/MS.
Material and methods. The flavonoid composition of sickle alfalfa was studied using qualitative reactions, paper chromatography, ultra performance liquid chromatography/MS on a Waters Acquility chromatograph with a UV diode array detector and a TQD tandem quadrupole MS detector (Waters) in the gradient elution mode. Flavonoid compounds were extracted with 70% ethyl alcohol.
Results. The flavonoid composition of the crescent alfalfa herb is represented by derivatives of quercetin, myricetin, apigenin, mainly their methoxylated forms. A total of 10 compounds were identified, including 8 flavonols, which were divided into quercetin derivatives (rutin, 4',7-O-Dimethylquercetin-3-O-di-methylglucoside, 4',7-O-dimethylquercetin-3-O-methylglucoside) , the rest are classified as myricetin derivatives. Two compounds are classified as flavones (apigenin-7-O-methylglucoside, apigenin-7-O-acetylglucosyl, glucosylglucoside).
Conclusion. 10 flavonoid compounds, represented mainly by methoxylated compounds, were identified in the herb alfalfa sickle, and therefore it can serve as a potential source of these compounds.
Keywords:
herba
sickle alfalfa
Medicago falcata L.
flavonoids
ultra performance liquid chromatography with photodiode array and tandem quadrupole mass selective detection.
References:
- Maevsky, P.F. Flora of the middle zone of the European part of Russia: 11th ed. M.: Association of Scientific Publications KMK, 2014; 157–9 (in Russian).
- Wild useful plants of Russia. St. Petersburg: Botan. in-t im. V.L. Komarova RAS: St. Petersburg. state chemical-pharmaceutical acad., 2001; 662 (in Russian).
- Javid T., Adnan M., Tariq A. et al. Antimicrobial activity of three medicinal plants (Artemisia indica, Medicago falcate and Tecoma stans). African J. of Traditional, Complementary and Alternative Medicines. 2015; 12 (3): 91−6. DOI:10.4314/ajtcam.v12i3.11
- Kiselevsky M.V., Babaskina L.I., Babaskin V.S. Study of the cytotoxic activity of extracts of various types of alfalfa. Far Eastern Medical J. 1997; 4: 73–5 (in Russian).
- Kovalev S.V. Chemical study of the lipophilic fraction of sickle alfalfa grass Zaporizhia Medical J. 2013; 3 (78): 94–7 (in Russian).
- Babaskina L.I. Pharmacognostic study of the genera alfalfa, lupine, vetch of the legume family, the prospect of their practical use: author. doctoral dissertations in pharmaceutical sciences. Moscow, 1998; 48 (in Russian).
- Grechana O.V. Aminoacid analysis of raw materials Medicago falcata L. subsp. romanica (Рrodan) O. Schwаrz & Klink. Naukovyy visnyk mizhnarodnogo humanitarnogo universitetu. 2014; 8: 50–2. DOI: 10.24959/nphj.14.1942
- Grechana O.V. Pharmacognostic study of yellow alfalfa (crescent or Romanian) Medicago falcata L. subsp. romanica (Рrodan) O. Schwarz & Klink”. Odessa Medical J. 2016; 2 (154): 24–8 (in Russian).
- Grechana O.V. The study of free coumarins in the plant raw material of medicago falcata l. Subsp. Romanica (prodan) O. Schwarz & klink. News of Pharmacy. 2014; 1 (77): 40–3. DOI: 10.24959/nphj.14.1942
- Andersen M., Markham K.R. Flavonoids. Chemistry, biochemistry and application: London-New York, 2006; 1198.
- Perova I.B., Eller K.I., Maltseva A.A., Chistyakova A.S., Slivkin A.I., Sorokina A.A. Flavonoids of the herb knotweed. Pharmacy. 2017; 2 (66): 15–9. DOI: https://doi.org/None (in Russian).
- Rodin M.N., Bokov D.O., Samylina I.A. Composition of biologically active compounds of herb succession. Pharmacy. 2022; 2(71):22-26. DOI: https://doi.org/10.29296/25419218-2022-02-04 (in Russian).
- Romanyuk A.A., Moiseev D.V. Hay leaves: component composition, pharmacological properties, standardization. Man and his health. 2021; 2 (24): 53–65. DOI: 10.21626/vestnik/2021-2/07 (in Russian)