Study of amino acid and elemental composition of dry extract of Samarkand immortelle

DOI: https://doi.org/10.29296/25419218-2022-08-06
Issue: 
8
Year: 
2022

E.S. Karieva, R.K. Sadikova, O.U. Karimov, F.Kh. Maksudova
Tashkent Pharmaceutical Institute, 45, Aibek str., Tashkent, 100015, Uzbekistan

Introduction. A comprehensive study of medicinal plants widely used in traditional medicine, in order to scientifically substantiate their use in practical medicine, is one of the urgent tasks of modern pharmacy. One of these plants is the Samarkand immortelle (Helichrysum maracandicum Popov ex Kirp.), widely distributed in Central Asia. Objectives. Study of the amino acid and elemental composition of the dry extract of Samarkand immortelle flowers. Material and methods. The elemental composition of the dry extract was determined by inductively coupled plasma mass spectrometry (ICP-MS), and the quantitative determination of interchangeable and essential amino acids was carried out by high-performance liquid chromatography. Results. In the dry extract of the Samarkand immortelle, the presence of 38 micro- and macroelements has been established, of which eight are vital (essential) and five are conditionally essential. Elements such as potassium, calcium, phosphorus, magnesium, sodium, aluminum, etc. are represented in the largest amount. According to the classification of Polyanskaya (2005), the detected elements by quantitative content can be divided into the following categories: • 2 macroelements (K, Ca); • 9 millielements (P, Mg, Na, Al, Fe, B, Mn, Zn, Cu); • 25 microelements (Ba, Cr, Ni, Ga, V, Li, Mo, Sr, Pb, Sc, Zr, Ag, Co, As, Ti, Tl, Se, Rb, Y, Sn, Sb, Cs, Be, Pt, Au); • 2 nanoelements (In, Cd). At the same time, the quantitative content of heavy metals and arsenic does not exceed the limits specified in regulatory documentation. Free amino acid analysis showed the presence of 20 amino acids in the dry extract, 10 of which are essential. The total free amino acid content is 6753.134 mcg/g, of which 29.19% are essential amino acids. Conclusion. The research results confirm the high value of the dry extract of the Samarkand immortelle in terms of the content of amino acids, macro- and microelements. This fact testifies to its supposed pharmacotherapeutic activity, possible breadth of application in medicine and the expediency of creating drugs based on it.
Keywords: 
dry extract
Samarkand immortelle
Helichrysum maracandicum Popov ex Kirp.
amino acid composition
elemental composition
High performance liquid chromatography method
Inductively coupled plasma mass spectrometry.
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