Development of approaches to standardization of the herb of the meadowsweet

DOI: https://doi.org/10.29296/25419218-2023-06-01
Issue: 
6
Year: 
2023

N.S. Sokolov, V.A. Kurkin, S.Kh. Sharipova, K.N. Sazanova
Samara State Medical University, Chapaevskaya ul., 89, Samara, 443099, Russian Federation

Introduction. Meadowseet Filipendula ulmaria (L.) Maxim. is widely distributed in the European part of Russia, in Western and Eastern Siberia. The plant has long been used in folk medicine, because it has a significant range of medicinal properties, many of which are associated with the presence of plant phenolic compounds, namely flavonoids. The purpose of this study is to develop a technique for quantifying the total of flavonoids in the herb of the meadowsweet, including optimization of the flavonoid extraction process using methods of mathematical planning of the experiment and spectrophotometric analysis of the extracts obtained. Material and methods. The material of the study was the herb of the meadowsweet, harvested in the period from 2020 to 2022 in the village of Alekseevka of the Samara region during the flowering phase (June–July). Biologically active compounds were extracted from plant raw materials, varying the main parameters of the extraction process in accordance with the selected experimental plan, followed by determining the conditions for maximum output product. The quantitative determination of the total of flavonoids in extracts from plant raw materials was carried out by differential spectrophotometry on the SF-2000 device (SDB Spectrum). Results. Optimal conditions for the extraction of flavonoids from raw materials were established: extractant – 40% ethyl alcohol, the ratio of "raw material-extractant" – 1:50, extraction time – 90 minutes. A method of quantitative determination of the total of flavonoids calculated on standard sample of rutin in the herb of the meadowsweet by differential spectrophotometry has been developed. It was determined that the content of the total of flavonoids in the herb of the meadowsweet varies from 3.90±0.02% to 4.02±0.04%. Conclusion. As a result of the conducted research, a technique for the quantitative determination of flavonoids in the herb of the meadowsweet has been developed, based on the optimization of the extraction process by the method of mathematical planning of the experiment and differential spectrophotometry of the extracts obtained.
Keywords: 
meadowsweet
Filipendula ulmaria (L.) Maxim.
herb flavonoids
rutin
spectrophotometry
standardization.
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