Anatomical aspects of Agastache rugosa (Fisch. et Mey.) Kuntze

DOI: https://doi.org/10.29296/25419218-2023-07-02
Issue: 
7
Year: 
2023

O.N. Shplis(1, 2), N.E. Kolomiets(1, 3)
1-Siberian State Medical University, Moskovsky trakt, 2, Tomsk, 634050, Russian Federation;
2-National Research Tomsk State University, Lenin St., 36, Tomsk, 634050, Russian Federation;
3-Kemerovo State Medical University, Voroshilova str., 22, Kemerovo, 650056, Russian Federation

Introduction. The samples of the Agastache rugosa (Fisch.et Mey.) Kuntze, cultivated in the conditions of the taiga zone of Western Siberia were study. Objective: determination of diagnostic microscopic (anatomical) signs of cultivated Agastache rugosa. Material and methods. Air-dry raw material of the Agastache rugosa, during the period of mass flowering and fruiting in 2021 were collected. The study of the anatomical structure, preparation and enlightenment of drugs was carried out in accordance with generally accepted methods. Leaf petioles and stems were fixed in 70% ethanol. Cross sections from each part of the stem (lower, middle, upper) were studied. The anatomical structure of the leaves was studied on the middle and terminal parts of the leaf, the epidermis of the petals upper and lower from the surface. All samples were studied in 5-fold repetition. Results. Specific features have been established: strongly winding lower leaf epidermis with characteristic thickened cell walls; 8-, rarely 6-cellular essential oil glands located in crypts; simple 1–4-celled and multicellular trichomes with a smooth or warty surface; capitate trichomes unicellular and bicellular on a unicellular, less often on a two-celled leg in leaves, flowers, petioles, less often in stems; papillary outgrowths of the epidermis of the stem, leaf, flowers. For the first time, receptacles, crystals of calcium oxalate in the form of drusen were found in the leaves, idioblast in the flowers, and an accumulation of small warty club-shaped bulbs. It has been established that in the leaves there are two types of stomata it is diacytic and anomocytic, in flowers it is only diacytic type. Conclusion. These specific data are important from the point of view of fundamental and practical interest, obtaining new data and diagnosing raw materials.

Keywords: 
Agastache rugosa
anomocytic and diacytic stomata type
receptacle
essential oil glands
drusen.

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