Anatomical aspects of Agastache rugosa (Fisch. et Mey.) Kuntze


O.N. Shplis(1, 2), N.E. Kolomiets(1, 3)
1-Siberian State Medical University, Moskovsky trakt, 2, Tomsk, 634050, Russian Federation;
2-National Research Tomsk State University, Lenin St., 36, Tomsk, 634050, Russian Federation;
3-Kemerovo State Medical University, Voroshilova str., 22, Kemerovo, 650056, Russian Federation

Introduction. The samples of the Agastache rugosa ( Mey.) Kuntze, cultivated in the conditions of the taiga zone of Western Siberia were study. Objective: determination of diagnostic microscopic (anatomical) signs of cultivated Agastache rugosa. Material and methods. Air-dry raw material of the Agastache rugosa, during the period of mass flowering and fruiting in 2021 were collected. The study of the anatomical structure, preparation and enlightenment of drugs was carried out in accordance with generally accepted methods. Leaf petioles and stems were fixed in 70% ethanol. Cross sections from each part of the stem (lower, middle, upper) were studied. The anatomical structure of the leaves was studied on the middle and terminal parts of the leaf, the epidermis of the petals upper and lower from the surface. All samples were studied in 5-fold repetition. Results. Specific features have been established: strongly winding lower leaf epidermis with characteristic thickened cell walls; 8-, rarely 6-cellular essential oil glands located in crypts; simple 1–4-celled and multicellular trichomes with a smooth or warty surface; capitate trichomes unicellular and bicellular on a unicellular, less often on a two-celled leg in leaves, flowers, petioles, less often in stems; papillary outgrowths of the epidermis of the stem, leaf, flowers. For the first time, receptacles, crystals of calcium oxalate in the form of drusen were found in the leaves, idioblast in the flowers, and an accumulation of small warty club-shaped bulbs. It has been established that in the leaves there are two types of stomata it is diacytic and anomocytic, in flowers it is only diacytic type. Conclusion. These specific data are important from the point of view of fundamental and practical interest, obtaining new data and diagnosing raw materials.

Agastache rugosa
anomocytic and diacytic stomata type
essential oil glands

  1. Plant resources of Russia: Wild flowering plants, their component composition and biological activity. Families Сaprifoliaceae-Lobeliaceae. СПб.–М., 2011; 4: 630 (in Russian).
  2. Wulf E.V. World resources of useful plants. Reference book. L.: Science, 1969; 562 (in Russian).
  3. Lavreneva G.V. Medicinal herbs: Herbs, that give health. Minsk, 1996; 33–4.
  4. Kolomiets N.E., Shplis O.N. Species of the genus Agastache J. Clayton ex Gronov.: distribution, use, degree of study (review). Chemistry of plant raw materials. 2022; 4: 5–26 (in Russian).
  5. Flora of the USSR. M.–L., 1954; 20: 556 (in Russian).
  6. Lim S.S. Chemical Composition of Essential Oils from Flower and Leaf of Korean Mint, Agastache rugosa. Asian J. of Chemistry, 2013; 25 (8): 4361–3. DOI:10.14233/ajchem.2013.13977
  7. Zielinska S. Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochemist Rew. 2014; 13 (2): 391–416. DOI:10.1007/s11101-014-9349-1
  8. Abdelaal H.A.A. The use of essential oils of traditional plants and a new plant for Russia – aniseed lofant [Lophanthus anisatus L. (Benth.)]. Natural Sciences. Astrakhan, 2009; 3 (28): 78–5 (in Russian).
  9. Khlebtsova E.B., Bokov D.O., Sorokina A.A. Anatomical study of the herb lofanta aniseed variety "Astrakhan 101". Pharmacy. 2013; 3: 31–34 (in Russian).
  10. Saparklycheva S.E. Chemical composition of aniseed lofant Lophanthus anisatus L. (Benth.)]. Agrarian education and science. 2019; 4: 16 (in Russian).
  11. Abramchuk A.V., Karpukhin M.Yu. Biomorphological features of Agastache Clayt ex Gronov, species in the conditions of the Middle Urals. Agrarian Bulletin of the Urals. 2016; 11 (153): 4–7 (in Russian).
  12. Rudnik G.O. Ontogenesis Agastache rugosa. Modern Phyto-morphology. 2013; 4: 257–60.
  13. Abramchuk A.V. Seedling method of cultivation of Tibetan lofant in the conditions of the Middle Urals. Agrarian Bulletin of the Urals. 2015; 11 (153): 293–7 (in Russian).
  14. Spiridovich.V., Fomenko T.I., Vlasov A.B., Mazur T.V., Yukhimuk A.N. Biotechnika. Pharmacy newspaper. 2012; 4 (58): 75–87 (in Russian).
  15. State Pharmacopoeia of the Republic of Belarus. Minsk, 2007; 2: 380–1.
  16. Chinese Virtual Herbarium(CVH): A National Taxonomic Database/ Institute of Botany, Academy of Sciences, Beijing, China, 100093. [Electronic edition]. Access mode: [Date of application: 27.07.2022]
  17. Medicinal Plants in the Republic of Korea. WHO Regional Publications Western Pacific Series. World Health Organization, Regional Office for the Western Pacific. Manila. 1998; 21: 318.
  18. Flora of Japan/By Jisaburo Ohwi. Edited by Frederick G. Meyer and Egbert H. Walker. Washington, D.C. Smithsonian Institution. 1965; 1067.
  19. Chinese Materia Medica. Chemistry, Pharmacology and Applications. Ping Y. Amsterdam. Harwood Academic Publishers.1998; 720.
  20. Wu Jing-Nuan An illustrated Chinese materia medica. Oxford [etc.]: Oxford univ. press, cop. 2005; 706. ISBN 0195140176 (alk. paper).
  21. Plenina L.V. Agastacin ® is a new plant hepatoprotector (results of clinical trials). Recipe. 2007; 2 (52): 132–6 (in Russian).
  22. Kim М., Woo T., Chung W., Kim Y., Lee J. The Effect of the Oil of Agastache rugosa O. Kuntze and Three of Its Components on Human Cancer Cell Lines. J. of Essential Oil Research. 2001; 13 (3): 214–8. DOI: 10.1080/10412905.2001.9699669
  23. Gong H., Li S., He L., Kasimu R. Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang. China. BMC Complementary and Alternative Medicine. 2017; 17 (1): 1–6. DOI: 10.1186/s12906-017-1605-7
  24. Kukhareva L.V., Tychina I.N., Kurchavaya A.I., Elyashevich A.V. Wrinkled mulberry (Lofant) (Agastache rugosa Fisch. et Mey) during introduction to Belarus. Spicy-aromatic and medicinal plants: prospects of introduction and use. Center. Bot. garden of NAS RB. Belarus. 1999; 71–2.
  25. Гуртовенко І.О., Коновалова О.Ю. Дослідження анатомічних діагностичних ознак сировини деяких видів роду Agastache як показників якості при стандартизації. Актуальні питання фармацевтичної і медичної науки та практики. 2018; 11, 2 (27): 230–7. DOI: 10.14739/2409-2932.2018.2.133179
  26. State Pharmacopoeia of the Russian Federation XIV edition. Volume 2. [Electronic edition]. Access mode: The State Pharmacopoeia оf The Russian Federation, XIV-ed. [Electronic resource]. Access mode: (in Russian).