Validation assessment of the method for the quantitative determination of phenolcarboxylic acids in the herb Phacelia tanacetifolia benth.


P.A. Sheykhmagomedova, T.T. Likhota, O.I. Popova
Pyatigorsk Medical and Pharmaceutical Institute – branch of Volgograd State Medical University, Kalinina Ave., 11, Pyatigorsk, 357532, Russian Federation

Introduction. Increasing the number of medicinal plant materials used is possible with the help of those plants that are widely used in folk medicine, and also, no less interesting for study are previously unexplored plants of foreign flora. Тansy-leaf phacelia is widely cultivated in our country as a honey plant. Phacelia honey has found its use in folk medicine. The chemical composition of the studied object is characterized by a predominant content of phenolic compounds: phenol carbonic acids, flavonoids, tannins, and anthocyanins. Among phenolic compounds, as the main group of the chemical composition of the herb Phacelia tansy, phenolcarboxylic acids dominate in content. For medicinal plants used in folk medicine, standardization of medicinal plant raw materials is carried out according to the main group of compounds. To assess the quality of the herb Phacelia tansy, a method has been developed for quantitative determination of the amount of phenolcarboxylic acids, at which the maximum yield of biologically active substances was observed. Purpose of the study. Conducting a validation assessment of the developed method for standardizing the herb Phacelia tansy. Material and methods. The object of the study is the herbs of Phacelia tanacetifolia Benth., grown and collected on the territory of the Republic of Dagestan in the vicinity of the city of Kaspiysk during the flowering period in April 2022. The grass was dried by air-shadow method. Results. Validation of the developed method was carried out according to the following indicators: specificity, linearity, accuracy and precision (repeatability). Based on the results obtained, it is clear that the validated method meets the appropriate acceptance criteria, which means it can be used to control the quality of the studied medicinal plant raw materials. Conclusion. Thus, the proposed method for quantitative determination of the amount of phenolcarboxylic acids in the herb Phacelia tansy is suitable for standardization for this indicator.

Тansy-leaf phacelia
Phacelia tanacetifolia Benth.
phenolcarboxylic acids
quantitative determination.

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