Study of flavonoids profile of linden flowers

DOI: https://doi.org/10.29296/25419218-2024-03-02
Issue: 
3
Year: 
2024

M.N. Rodin(1), D.O. Bokov(1, 2), N.B. Lazareva(1)
1-Sechenov First Moscow State Medical University (Sechenov University), st. Trubetskaya, 8, bldg. 2, Moscow, 119991, Russian Federation;
2-Federal State Budgetary Scientific Institution “Federal Research Center of Nutrition and Biotechnology”, Ustinsky proezd, building 2/14, Moscow, 109240, Russian Federation

Introduction. Linden flowers are pharmacopoeial medicinal plant raw materials. It is widely used in clinical practice for the treatment and prevention of respiratory diseases. Despite the high degree of scientific study of this MPRMs, the improvement of approaches to standardization remains a pressing issue. Objective: study the composition of flavonoids of industrially produced linden flowers, presented on the Russian pharmaceutical market. Material and methods. Ultra-high-performance liquid chromatography with photodiode array and tandem quadrupole mass-selective detection in the gradient elution mode was used to analyze flavonoids in industrial samples of linden flowers. Extraction was performed with 70% aqueous methanol in a water bath. Results. The presence of flavonoids characteristic for linden flowers was confirmed. 13 flavonoids were identified (quercetin-3-rutinosid-7-rhamnoside, quercetin-3-glucoside-7-rhamnoside, tiliroside, rutin, quercetin-3-glucoside, kaempferitrin, kaempferol 3-rutinosid, quercetin-3-arabinoside, astragalin, quercetin 3-rhamnoside, kaempferol 7-rhamnoside, quercetin 3-glucuronide, prunetin-4'-rutinosid). Conclusion. During the experiment, a flavonoid profile was identified and it is consistent with literature data. When standardizing linden flowers and medicinal herbal preparations based on them, it is recommended to use quercetin and kaempferol derivatives as marker compounds. These compounds are specific and the inclusion of a definition of this group of biologically active compounds in the RD to confirm authenticity seems very relevant when standardizing linden flowers.
Keywords: 
Linden flowers
flavonoids
ultra-performance liquid chromatography with photodiode array and tandem quadrupole mass selective detection method
standardization.
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