Identification of phenolic compounds in Artemisia scoparia

DOI: https://doi.org/10.29296/25419218-2024-04-02
Issue: 
4
Year: 
2024

E.E. Ayrapetyan, M.V. Larsky, D.A. Konovalov
Pyatigorsk Medical and Pharmaceutical Institute is a branch of the Volgograd State Medical University of the Ministry of Health, Kalinin Ave., 11, Pyatigorsk, 357532, Russian Federation

Introduction. Artemisia scoparia herba is a valuable source of biologically active compounds. Due to its rich chemical composition, the raw material has wide pharmacological effects. Purpose of the study. Identification of some phenolic compounds and the quantitative determination of scoparone, scopoletin, umbelliferonein Artemisia scoparia herba by high performance liquid chromatography (HPLC). Material and methods. The investigated object is Artemisia scoparia herba, collected during the flowering phase in the Nikitsky Botanical Garden in 2018–2019. Qualitative determination of phenolic compounds was provided by HPLC with mass-spectrometry detection (HPLC-MS). Results. The following phenolic compounds were identified by HPLC-MS: scopoletin, rutin, hyperoside, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, luteolin. Conclusion. The possibility of qualitative and quantitative determination of phenolic compounds from plant raw materials by HPLC is shown.
Keywords: 
Artemisia scoparia Waldst. et Kit.
phenolic compounds
scoparon
scopoletin
umbelliferone
HPLC-MS.
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