Comparative research of the elemental composition of red Clover (Trifolium pratense L.) and middle Clover (Trifolium medium L.)

DOI: https://doi.org/10.29296/25419218-2024-04-03
Issue: 
4
Year: 
2024

А.V. Nikulin(1), F.V. Radus(1), Yu.А. Efimova(1), T.Yu. Kovaleva(2), O.G. Potanina(3)
1-MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies), Vernadsky Avenue, 78, Moscow, 119454, Russian Federation;
2-Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya str., 8/2, Mosсow, 119991, Russian Federation
3-Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119991, Russian Federation

Introduction. Medicinal plants are currently of increased interest. However, the deterioration of the environmental situation significantly complicates the conduct of resource research. At the same time, some types of medicinal plant raw materials can be natural concentrators of elements, which makes it possible to use herb as natural sources of essential minerals. One of these types of raw materials is herbs of red clover and medium clover. Objective: Study of the elemental composition of meadow clover and medium clover herbs collected in two districts of the Moscow region. Material and methods. Trace elements were determined using an inductively coupled plasma atomic emission spectrometer (ICP-AES) Avio 200 by Perkin Elmer, normalized heavy metals using an atomic absorption spectrometer with electrothermal atomization (ETAAS). Results. 10 trace elements were determined in the studied samples: of these, the content of Al, B, Ba, Fe, Mn, Sr, Zn is on average at the level of 10-3 %, Ca, Mg, K, P, S at the level of 0.2-1.0 %. The content of Ag, Be, Bi, Co, Cr, Cu, Li, Mo, Ni, Sb, Sn, Ti, V, Zr was at the detection limit and didn’t exceed 10-4 %, Cd, Pb, As, Hg were not detected in the analyzed samples. Conclusion. It was found that the analyzed samples have a similar elemental composition. Taldomsky and Losino-Petrovsky districts of the Moscow region can be considered as potential places for collecting raw materials. However, the studied herb cannot be considered as a potential source of trace elements.
Keywords: 
red clover
medium clover
herbs
inductively coupled plasma atomic emission spectrometer
atomic absorption spectrometer with electrothermal atomization.
References: 
  1. Новиков В.С., Шустов Е.Б. Роль минеральных веществ и микроэлементов в сохранении здоровья человека. Вестник образования и развития науки Российской академии естественных наук. 2017; 21 (3): 5–16. [Novikov V.S., Shustov E.B. Role of minerals and microelements in human health maintenance. Vestnik obrazovaniya i razvitiya nauki Rossijskoj akademii estestvennyh nauk. 2017; 21 (3): 5–16 (In Russian)].
  2. Gharibzahedi S. M. T., Jafari S. M. The importance of minerals in human nutrition: Bioavailability, food fortification, processing effects and nanoencapsulation. Trends in Food Science & Technology. 2017; 62: 119–32. DOI: 10.1016/j.tifs.2017.02.017
  3. Гольдфейн М.Д., Адаев О.Н., Тимуш Л.Г., Заиков Г.Е., Ярошевская Х.М. Роль химических элементов и их соединений в природе и в процессах жизнедеятельности человека Часть 1. химические вещества в экологии, микроэлементозы и общие вопросы безопасности питания. Вестник Казанского технологического университета. 2015; 18 (16): 296–300. [Goldfein M.D., Adaev O.N., Timush L.G., Zaikov G.E., Yaroshevskaya H.M. The role of chemical elements and their compounds in nature and in human life processes part 1. Chemicals in ecology, trace elements and general food safety issues. Vestnik Kazanskogo tekhnologicheskogo universiteta. 2015; 18 (16): 296–300 (In Russian)].
  4. Коробко И.Ю., Журня А.А., Мельникова О.П., Павлова О.С. Микро- и макроэлементы и сердечно-сосудистые заболевания. Кардиология в Беларуси. 2018; 10 (2): 280–9. [Korobko I., Zhurnia H., Melnikova O., Pavlova O. Microelements and macroelements and cardiovascular disease. Kardiologiya v Belarusi. 2018; 10 (2): 280–9 (In Russian)].
  5. Zgórka, G., Maciejewska-Turska, M. Cultivation and Utilization of Red Clover (Trifolium pratense L.). Medicinal Plants: Domestication, Biotechnology and Regional Importance. 2021; 28: 315–38. https://doi.org/10.1007/978-3-030-74779-4_10.
  6. Сюткина А.И. Трава клевера как перспективный источник лекарственного растительного сырья. Молодой ученый. 2015; 11 (91): 719–21. [Syutkina A.I. Clover grass as a promising source of medicinal plant raw materials. Molodoj uchenyj. 2015; 11 (91): 719–21 (In Russian)].
  7. Ефремов А.П. Лекарственные растения и грибы средней полосы России. М.: Фитогон XXI, 2018; 504. [Efremov A.P. Medicinal plants and fungi of Central Russia. M.: Fitogon XXI, 2018; 504 (In Russian)].
  8. . Головкин Б.Н., Руденская Р.Н., Трофимова И.А., Шретер А.И. Биологически активные вещества растительного происхождения. М.: Наука, 2001; 1: 350. [Golovkin B.N., Rudenskaya R.N., Trofimova I.A., SHreter A.I. Biologically active substances of plant origin. M.: Nauka, 2001; 1: 350 (in Russian)].
  9. Butkutė B., Lemežienė N., Padarauskas A., Norkevičienė E., Taujenis L. Chemical Composition of Zigzag Clover (Trifolium medium L.). Breeding Grasses and Protein Crops in the Era of Genomics. 2018; 83–7. https://doi.org/10.1007/978-3-319-89578-9_15
  10. Андреева В.Ю., Калинкина Г.И., Полуэктова Т.В., Гуляева В.А. Сравнительное исследование фенольных соединений видов рода клевер (Trifolium L.) флоры Сибири. Химия растительного сырья. 2018; 1: 97–104. DOI 10.14258/jcprm.2018011846. [Andreeva V.Yu., Kalinkina G.I., Poluektova T.V., Gulyaeva V.A. The comparative study of phenolic compounds in Trifolium L. species in Siberia. Himiya rastitel'nogo syr'ya. 2018; 1: 97–104. DOI 10.14258/jcprm.2018011846 (In Russian)].
  11. Нгуен Т.Ш., Дитковская З.Р., Генералова Ю.Э., Каухова И.Е., Сорокин В.В. Определение элементного состава травы клевера лугового (Trifolium pratense L.). Вопросы биологической, медицинской и фармацевтической химии. 2020; 23 (2): 51–5. DOI: 10.29296/25877313-2020-02-08. [Nguyen T.S., Ditkovskaya Z.R., Generalova Y.E., Kaukhova I.E., Sorokin V.V. Determination of the mineral element composition of red clover grass (Trifolium pratense L.). Voprosy biologicheskoi, meditsinskoi i farmatsevticheskoi khimii. 2020; 23 (2): 51-5. DOI: 10.29296/25877313-2020-02-08 (In Russian)].
  12. Жалолов И.Ж., Aбдурахмонова С.Б. Исследование макро и микроэлементов растения Trifolium pratense методом ICP-MS. Universum: химия и биология. 2023; 2 (104). DOI:10.32743/UniChem.2023.104.2.14909. [Jalolov I., Abdurahmonova S. Investigation of macro and micro elements of the plant trifolium pratense by the ICP-MS method. Universum: khimiya i biologiya. 2023; 2 (104). DOI: 10.32743/UniChem.2023.104.2.14909 (In Russian)].
  13. Государственная фармакопея РФ XV издание [Электронное издание]. Режим доступа: https://pharmacopoeia.regmed.ru/ pharmacopoeia/izdanie-15/. [Дата обращения: 30.10.2023]. [The State Pharmacopoeia of The Russian Federation, XV ed. [Electronic edition]. Access mode: https://pharmacopoeia.regmed.ru/ pharmacopoeia/izdanie-15/. [Accessed 30 October 2023] (in Russian)].
  14. Алексеев А. В., Якимович П.В., Кваченок И.К. Определение примесей в никеле методом ИСП-МС. Труды ВИАМ. 2020; 2 (86): 101–8. DOI 10.18577/2307–6046-2020-0-2-101-108. [Alekseev A.V., Yakimovich P.V., Kvachonok I.K. Determination of impurities in nickel by ICP-MS. Trudy VIAM. 2020; 2 (86): 101–8. DOI 10.18577/2307–6046-2020-0-2-101-108 (In Russian)].
  15. Дворецков Р.М., Карачевцев Ф.Н., Демин С.А. Аналитический контроль химического состава гальванических электролитов никелирования методами АЭС-ИСП и МС-ИСП. Труды ВИАМ. 2023; 3 (121): 117–31. DOI: 10.18577/2307–6046-2023-0-3-117-131. [Dvoretskov R.M., Karachevtsev F.N., Demin S.A. Analytical control of the chemical composition of galvanic electrolytes of nickel plating by the ICP-AES and ICP-MS methods. Trudy VIAM. 2023; 3 (121): 117–31. DOI 10.18577/2307–6046-2023-0-3-117-131 (In Russian)].
  16. Planeta K., Kubala-Kukus A., Drozdz, A. The assessment of the usability of selected instrumental techniques for the elemental analysis of biomedical samples. Scientific Reports. 2021; 11 (3704). https://doi.org/10.1038/s41598-021-82179-3