FLAVONOIDS OF LADY’S THUMB (POLYGONUM PERSICARIA) HERB

DOI: https://doi.org/None
Issue: 
2
Year: 
2017

I.B. Perova (1), PhD; Professor K.I. Eller (1), PhD; A.A. Maltseva (2), PhD; A.S. Chistyakova (2); Professor A.I. Slivkin (2), PhD; Professor A.A. Sorokina (3), PhD 1-Federal Research Institute of Nutrition, Biotechnology, and Food Safety; 2/14, Ustyinsky Proezd, Moscow 109240, Russian Federation; 2-Voronezh State University; 1, Universitetskaya Sq. Voronezh 394006, Russian Federation; 3-I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

Introduction. Lady’s thumb (Polygonium persicaria) is widely distributed in Central Russia. The plant is rich in biologically active substances and has long been used in medical practice. The data available in the literary on the composition of flavonoids in the lady’s thumb herb are often contradictory. Objective: to investigate the profile of flavonoids in the lady’s thumb herb harvested in the Voronezh Region. Material and methods. The investigation object was lady’s thumb herb grown in the Voronezh Region. Preliminary studies were performed by thin-layer chromatography (TLC). The composition and content of flavonoids were determined by high performance liquid chromatography (HPLC) with UV diode array and time-of-flight mass spectrometric detection (HPLC-UV/MS) using an Agilent 1100 system. Results. The areas of flavonoid substances were observed mainly in the aqueous-alcohol extracts. These were not seen in the heptanoic extracts characteristic of flavonoids. The lady’s thumb was ascertained to contain 27 derivatives of flavonols and flavones. Sixteen compounds were identified. The total content of flavonoids in the lady’s thumb herb amounted to 12.05 mg/g. Conclusion. A profile of lady’s thumb flavonoids was established, which included O- and C-glycosides of kaempferol, quercetin, apigenin, and luteolin.

Keywords: 
lady’s thumb
Polygonum persicaria L.
herb
flavonoids
thin-layer chromatography
high-performance liquid chromatography - mass spectrometry

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