QUANTITATIVE DETERMINATION OF AMINO ACIDS IN THE DRIED BLOOD OF MARAL (CERVUS ELAPHUS): VALIDATION OF HPLC PROCEDURE

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DOI: https://doi.org/None
Issue: 
4
Year: 
2017

K.P. Lunin; Professor V.F. Turetskova, PhD; O.G. Makarova, PhD Altai State Medical University; 40, Lenin Pr., Barnaul 656038, Russian Federation

Introduction. The adaptogenic activity of the dried blood of maral (Cervus elaphus) is associated with amino acids. The qualitative and quantitative compositions of amino acids in the above substance are analyzed rarely and using obsolete techniques. Objective: to estimate the validation of the developed HPLC procedure for quantifying the main amino acids (alanine, leucine, and lysine) in the dried maral blood. Material and methods. The object of the investigation included dried maral blood. Native blood from stags was sampled when cutting their antlers in May to June 2014. The investigators developed a procedure based on the cleavage of peptide bonds in hydrochloric acid with the subsequent modification of the resulting amino acids by phenylisothiocyanate and by the further HPLC separation of derivatives of amino acids. The validation of the procedure was assessed using the following characteristics: specificity, linearity, suitability, correctness, and precision during repeatability. Results. It was found that the presence of accessory agents did not affect the results of an analysis. Spectral ratios, retention times, and absorption maxima of the substances analyzed in the test and standard samples were within the acceptance limits. The correctness and suitability of procedure during repeatability was established; a linear relationship between the magnitude of an analytical signal and the amount of a detectable substance was found. Conclusion. The procedure for HPLC quantification of main maral blood amino acids, such as alanine, leucine, and lysine, corresponds to the recommended validation values and may be used to assess the quality of dried maral blood
Keywords: 
dried maral blood
pantohematogen
validation
amino acids
References: 
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