INVESTIGATION OF PHENOLIC COMPOUNDS IN HOMEOPATHIC INDIGO (BAPTISIA) TINCTURES

DOI: https://doi.org/None
Issue: 
4
Year: 
2017

D.A. Nikishin (1), N.S. Teryoshina (1), V.N. Tashlitsky (2), 1-I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 19991, Russian Federation; 2-Faculty of Chemistry, M.V. Lomonosov Moscow State University; 1, Leninsky Gory, Build. 3, GSP-1, Moscow 119991, Russian Federation

Introduction. Wild indigo (Baptisia tinctoria) roots are used in medicine mainly to treat infectious diseases and cancer. The raw material contains quinolizidine alkaloids, phenolic compounds, coumarins, tannins, triterpene saponins, glycoproteins, and polysaccharides. Objective: to comparatively investigate homeopathic matrix tinctures of indigo roots. Material and methods. Homeopathic tinctures from fresh and dried indigo roots were matters for this investigation that was conducted by high performance liquid chromatography using a chromatograph. Results. The homeopathic indigo roots were found to contain 18 phenolic compounds. Ten compounds were admittedly identified by retention times and mass spectra. Conclusion. Homeopathic matrix tinctures from fresh and dried indigo roots have different qualitative and quantitative phenolic compositions.

Keywords: 
wild indigo
Baptisia tincture (L.) R.Br.
homeopathic tincture
phenolic compounds
flavonoids
high performance liquid chromatography

References: 
  1. Classen B., Thude S., Blaschek W.et al. Immunomodulatory effects of arabinogalactan-proteins from Baptisia and Echinacea. Phytomedicine, 2006; 13: 688–94.
  2. Homeopathy Baptisia Wild Indigo. Rezhim dostupa: http://www.herbs2000.com/homeopathy/baptisia.htm.
  3. Sandoval C.H., Morfin L.L., Lopez B.B. Preliminary research for testing Baptisia tinctoria 30c effectiveness against salmonellosis in first and second quality broiler chickens. British Homoeopathic Journal, 1998; 87: 131–4.
  4. Patudin A.V., Teryoshina N.S., Mishhenko V.S., Il`enko L.I. Biologicheski aktivnye veshhestva gomeopaticheskogo lekarstvennogo syr`ya. M.: Znak, 2009; 588. [Patudin A.V., Teryoshina N.S., Mishenko V.S., Ilienko L.I. Biologically active substances of homeopathic medicinal raw materials. Moscow: Znak, 2009; 588] (in Russian).
  5. Alston R.E. Flavonoid Chemistry of Baptisia: A Current Evaluation of Chemical Methods in the Analysis of Interspecific Hybridization. Taxon, 1965; 14 (8): 268–74.
  6. Harborne J.B., Baxter H., Moss G.P. Phytochemical dictionary: a handbook of bioactive compounds from plants. London: Taylor & Francis Ltd, 1999; 961.
  7. Bajaj Y.P.S. Biotechnology in Agriculture and Forestry 28. Medicinal and Aromatic Plants VII. Springer Science & Business Media; 2013: 475.
  8. Udayama M., Kinjo J., Nohara T. Triterpenoidal saponins from Baptisia australis. Phytochemistry, 1998; 48 (7): 1233–5.
  9. Wack M., Classen B., Blaschek W. An acidic arabinogalactan – protein from the roots of Baptisia tinctoria. Planta Medica, 2005; 71 (9): 814–8.
  10. Harnischfeger G., Stolze H. Bewährte Pflanzendrogen in Wissenschaft und Medizin. Notamed Verlag, Bad Homburg: Melsungen, 1983; 296.
  11. Comparison of total phenolic content in Baptisia tinctoria root and indigowoad root from different origin. Rezhim dostupa: http://www.global-summit.com/speaker-pdfs/2014/wai-kun-chan-national-i-lan-university-taiwan.pdf.
  12. Golovkin B. N. i dr. Biologicheski aktivnye veshhestva rastitel`nogo proishozhdeniya. Maakiain (maackiain). M.: Nauka, 2001; 764. [Golovkin B.N. et al. Biologically active substances of plant origin. Maackiain. Moscow: Nauka, 2001; 764] (in Russian).
  13. Shukla S., Bhaskaran N., Babcook M.A. et al. Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway. Carcinogenesis,. 2014; 35 (2): 452–60.