USE OF HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY FOR THE SEPARATION OF POSITIONAL BUTYLHYDROXYANISOLE AND ISOSORBIDE MONONITRATE ISOMERS

DOI: https://doi.org/None
Issue: 
5
Year: 
2017

A.S. Osipov, PhD; O.A. Popova; S.G. Larionova, PhD; T.N. Gretskaya, PhD; S.E. Milkina Research Center for Examination of Medical Products, Ministry of Health of the Russian Federation; 6, Shchukinskaya St., Moscow 123182, Russian Federation

Introduction. A mixture of butylhydroxyanisole (BHA) and isosorbide mononitrate (ISMN) isomers should be used to study the properties of chromatographic columns. Objective: to investigate whether chromatographic columns packed with amide, diol, and amino sorbents for hydrophilic interaction liquid chromatography can be used to separate BHA and ISMN isomers. Material and methods. Standard samples of butylhydroxytoluene and BHA (a mixture of isomers), isosorbide dinitrate, isosorbide-2-nitrate, and isosorbide-5-nitrate were used. Columns packed with diol, amino, and amide sorbents used for hydrophilic interaction liquid chromatography were studied. Results. The authors obtained experimental data on the separation of BHA and ISMN isomers using different columns. The columns containing diol sorbents failed to separate BHA isomers when hydrophilic interaction chromatography was employed. The compounds in question were badly separated when a column containing an amide-sorbent was used. Conclusion. All isomers can be separated by hydrophilic interaction chromatography using amino-sorbent columns containing acetonitrile in the mobile phase greater than 99%.

Keywords: 
high performance liquid chromatography
butylhydroxyanisole
isosorbide dinitrate
isosorbide mononitrate

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