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E.P. Tsatsua (1), A.P. Astashkina (2), PhD; Professor V.K. Shormanov (1), PhD; M.V. Rymarova (1), PhD; A.O. Kvasova (1) 1-Kursk State Medical University; 3, K. Marx St., Kursk 305041, Russian Federation 2-Tomsk National Research Polytechnic University; 30, Lenin Pr., Tomsk 634050, Russian Federation

Introduction. 2,6-Di-tert-butylhydroxybenzene (2,6-DTBHB) is a substance that has antioxidant and antiradiation activities and is used as an antioxidant and an intermediate for organic syntheses. The compound is toxic to warm-blooded animals; cases of human poisoning have been recorded. Objective: to investigate the features of determination of 2,6-DTBHB in liver tissues and blood. Material and methods. The investigation object was model mixtures of 2,6-DTBHB and liver tissue. Thin-layer chromatography (TLC), ultraviolet (UV) and infrared (IR) spectrophotometries, and a reaction to obtain an acy-nitro derivative were used for their identification and quantification. Results. The investigation showed how the compound in question could be purified from the endogenous substances of biological matrices by adsorption chromatography in a column packed with silica gel L 40/100 µm, eluting with a mixture of hexane:dioxane (97,5:2,5). Procedures were developed to identify and quantify 2,6-DTBHB in the extracts from biological material, by applying TLC, IR and UV spectrophotometry. The optimal parameters of nitration of 2,6-DTBHB and subsequent transfer of the resultant nitro derivative to stained acy-nitro salt were established. Conclusion. A scheme for examining organ tissues and blood was proposed to confirm that they contain 2,6-DTBHB.

chromatographic purification

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