HYDROXYCINNAMIC ACIDS OF LADY’S THUMB (POLYGONUM PERSICARIA) HERB

Download full text PDF
DOI: https://doi.org/None
Issue: 
5
Year: 
2017

I.B. Perova (1), PhD; Professor K.I. Eller (1), PhD; A.A. Maltseva (2), PhD; A.S. Chistyakova (2); Professor A.I. Slivkin (2), PhD; Professor A.A. Sorokina (3), PhD 1-Federal Research Center of Nutrition, Biotechnology, and Food Safety; 2/14, Ustyinsky Proezd, Moscow 109240, Russian Federation; 2-Voronezh State University; 1, Universitetskaya Sq. Voronezh 394006, Russian Federation; 3-I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 119991, Russian Federation

Introduction. Lady’s thumb (Polygonum persicaria) contains biologically active substances, predominantly phenolic compounds, including hydroxycinnamic acids. The latter make a considerable contribution to the therapeutic effect of combined herbal medicines due to their diverse biological activities. Objective: to investigate the profile of hydroxycinnamic acids in the lady’s thumb herb. Material and methods. The investigation object was lady’s thumb herb grown in the Voronezh Region. The composition and content of hydroxycinnamic acids were determined by spectrometry by means of a SP 2000-01 device and by high performance liquid chromatography (HPLC) with UV diode array and time-of-flight mass spectrometric detection (HPLC-UV/MS) using an Agilent 1100 system. Results. The UV spectrum of extraction from lady’s thumb herb showed the maximum absorption as a shoulder at 320-350 nm and had spectral characteristics similar to those of chlorogenic acid. The raw material content of a sum of hydroxycinnamic acids calculated with reference to chlorogenic acid was 1.95%. HPLC-UV/MS revealed that lady’s thumb herb contained 7 hydroxycinnamic acid derivatives; 2 compounds were identified as chlorogenic and cryptochlorogenic acids. Conclusion. The profile and content of hydroxycinnamic acids in the herb of lady’s thumb grown in the Voronezh Region have been established, which is represented by 7 compounds.
Keywords: 
lady’s thumb
Polygonum persicaria L.
hydroxycinnamic acids
ultraviolet spectrophotometry
high-performance liquid chromatography – ultraviolet mass spectrometry
References: 
  1. Maevskiy P.F. Flora sredney polosy Evropeyskoy chasti Rossii. 11-e izd. M.: Tovarishhestvo nauchnyh izdaniy KMK, 2014; 635.
  2. Burda N.E. i dr. Kolichestvennoe opredelenie gidroksikorichnyh kislot v trave i podzemnyh organah Filipendula ulmaria (L.) Maxim. Materialy Vserossiyskoy s mezhdunarodnym uchastiem nauchno-prakticheskoy konferencii «Tradicii i innovacii farmacevticheskoy nauki i praktiki». Kursk (aprel`, 2011); 209–12.
  3. Zaporozhec O.A. i dr. Spektrofotometricheskoe opredelenie gidroksikorichnoy kisloty i ee proizvodnyh v preparatah e`hinacei. Himiko-farmacevticheskiy zhurnal, 2003; 32 (12): 11–4.
  4. Gosudarstvennaya farmakopeya RF XIII izd. [E`lektronnyy resurs]. Rezhim dostupa: http://www.femb.ru/feml Data obrashheniya: 19.11.2016.
  5. Zhogova A.A. i dr. Identifikaciya i kolichestvennoe opredelenie osnovnyh biologicheski aktivnyh veshhestv travy pustyrnika s pomoshh`yu VE`ZhH-mass-spektrometrii. Himiko-farmacevticheskiy zhurnal, 2014; 48 (7): 54–9.
  6. Zhukova O.L. i dr. Izuchenie fenol`nogo sostava podzemnyh organov sabel`nika bolotnogo. Vest. Moskovskogo universiteta. Ser.2. Himiya, 2006; 47(5): 342–5.