TRITERPENIC SAPONINS IN THE LEAVES AND BUDS OF BLACKCURRANTS (RIBES NIGRUM L.)

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DOI: https://doi.org/None
Issue: 
8
Year: 
2015

T.S. Popova; N.S. Teryoshina, PhD I.M. Sechenov First Moscow State Medical University; 8, Trubetskaya St., Build. 2, Moscow 19991

Much attention has been recently given to the estimation of saponins in medicinal plant material, as triterpenic saponins have a negative effect on humans. To determine this group of biologically active substances in the leaves and buds of blackcurrants (Ribes nigrum L.), a procedure has been developed for the spectrophotometric determination of the sum of triterpenic saponins calculated with reference to escin, by carrying out a color reaction with sulfuric acid. The sensitivity of the method is 0.05 mg/ml; a linear relationship is observed in the range of 0.0005 to 0.004%. The relative error of determination at 0.95 confidence probability is not greater than ±4.0%. The content of triterpenic saponins in the buds and leaves of blackcurrants is 2.01–2.22 and 2.81–3.39%, respectively.
Keywords: 
blackcurrants (Ribes nigrum L.)
leaves
buds
triterpenic saponins
spectrophotometry
assay
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